Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1978-06-02
1980-11-18
Fagelson, Anna P.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 1, 424 11, 424 12, 424 13, G01N 3122, G01N 2152, G01N 3316
Patent
active
042345634
ABSTRACT:
A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described. The method comprises incubating an insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) or other substrates with serum from patients with systemic lupus erythematosus (SLE) or other autoimmune diseases for a sufficient length of time, washing the precipitate of flocculate, separating and discarding therefrom the supernatant fluid, adding a fluorescein labeled anti-immunoglobulin antibody to the washed flocculate and incubating the labeled mixture for a sufficient length of time, washing the incubated flocculate and separating therefrom the supernatant fluid, suspending the pellet in a liquid medium to determine the fluorescence of the flocculate, which is proportional to the concentration of antibodies to deoxyribonucleic acid and others in serum specimen. The insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) retains the double-stranded form of native deoxyribonucleic acid (n-DNA).
REFERENCES:
patent: 3992631 (1976-11-01), Harte
Steinman, The J. of Clin. Invest. vol. 57, May, 1976 pp. 1330-1341.
Lerman, Biochem & Biophys Acta, vol. 18, 1955 pp. 132-134.
Tan, J. Lab. & Clin. Med., vol. 81, Jan. 1973 pp. 122-132.
Miles Labs, Research Products, Cat. C, Miles Labs. Kankakee, Ill. p. 24.
Sharp, J. Lab & Clin. Med., Vol. 74, Dec. 1969, Ab. No. 160 pp. 1010-1011.
American Hospital Supply Corporation
Fagelson Anna P.
Figg Edward A.
Hartenberger Robert E.
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