Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-22
1995-10-17
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 4, 436 56, 436 73, 436 77, 436 79, 436 80, 436 81, 436 83, 436 84, 436501, G01N 3320, G01N, G01N, G01N 3358
Patent
active
054590400
ABSTRACT:
Methods of assaying for the presence or amount of a metal ion in a sample suspected of containing such ions. In one aspect, an enzyme amplified sandwich assay is provided which relies upon the ability of the analyte (metal ion) to form a complex with two complexing agents (chelators). In this assay, the first sandwich chelator is immobilized on a solid support, while the second sandwich chelator is linked to a reporter group (e.g., an enzyme). This assay combines the specific recognition of the analyte by the first and second sandwich chelators with the great signal amplification offered by the reporter group (e.g., enzyme). In another aspect, a competitive assay is provided that relies on the competitive inhibition of complex formation between the coating ligand (i.e., the chelator attached to the solid support) and the organometallic compound attached to the reporter group (e.g., enzyme) by the metal ions of interest present in the sample.
Using the methods of the present invention, hazardous metals, environmental pollutants, and other biomolecules can be selectively detected at ppb/ppt concentrations. More particularly, using the methods of the present invention, highly toxic metals (mercury, lead, cadmium, etc.) that pose serious human and environmental health hazards can be selectively detected at ppb/ppt concentrations. The methods of the present invention are particularly useful because they do not require the use of high affinity, highly specific antibodies which are expensive and difficult to produce.
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Hammock Bruce D.
Kido Horacio
Szurdoki Ferenc
Saunders David
The Regents of the University of California
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