Method for synthesizing single-stranded stem-loop DNAs, the prod

Details Method for synthesizing single-stranded stem-loop DNAs, the prod Method for synthesizing single-stranded stem-loop DNAs, the prod

1994-08-02

1997-07-01

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 4351723, 536 231, 536 253, C12P 1934, C12N 1511

Patent

active

056437625

ABSTRACT:
The invention provides a method for synthesizing new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or in vitro synthesis. Replicating vehicles are provided which produce these ss-slDNAs. The slDNAs can be used for introducing random mutations in a selected gene, and they lend themselves for replication by a variant of the PCR method.

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