Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-07-10
1997-07-01
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 73, 435 732, 436518, G01N 3353, G01N 33543, G01N 33554, G01N 33569
Patent
active
056437331
ABSTRACT:
This invention relates generally to an assay for Lyme disease which detects the antibody to Borrelia burgdorferi, the causative agent of Lyme disease. More specifically, the assay employs antigens derived from amino acid regions in the flagellum of Borrelia burgdorferi. These antigens are immunoreactive with antibodies to Borrelia burgdorferi but are not substantially immunoreactive with antibodies to Treponema pallidum, the syphilis causing agent. DNA sequences of the antigens, clones and vectors containing the DNA sequences are also disclosed. Polypeptides derived therefrom can be used as reagents for the detection of antibody to Borrelia burgdorferi in the body fluids from individuals with Lyme disease.
REFERENCES:
Journal of Bacteriology, vol. 173, No. 4, Feb. '91, G. S. Gassmann et al., "Analysis of the Borrelia burgdorferi GeHo fla Gene and Antigenic Characterization of Its Gene Product" pp. 1452-1459.
Infection and Immunity, vol. 52, No. 5, May 1986, A.G. Barbour et al., "A Borrelia-Specific Monoclonal Antibody Binds to a Flagellar Epitope", pp. 549-554.
Nucleic Acids Research, vol. 17, No. 9, 1989, G.S. Gassmann et al., "Nucleotide sequence of a gene encoding the Borrelia burgdorferi Flagellin", pp. 3590-3535.
Infection and Immunity, vol. 59, No. 10, Oct. 1991, R. Berland, et al., "Molecular Characterization of the Humoral Response to the 41-Kilodalton Flagellar Antigen of Borrelia burgdorferi, the Lyme Disease Agent", pp. 3531-3535.
Infection and Immunity, vol. 58, No. 6, Jun. 1990, R. Wallich et al., "The Borrelia burgdorferi Flagellum-Associated 41-Kilodalton Antigen (Flagellin): Molecular Cloning, Expression, and Amplification of the Gene", pp. 1711-1719.
Infection and Immunity, vol. 59, No. 2, Feb. 1991, C. Collins et al., "Immunoreactive Epitopes on an Expressed Recombinant Flagellar Protein of Borrelia burgdorferi", pp. 514-520.
E. Harlow, et al., Antibodies A Laboratory Manual, Cold Spring Harbor Laboratory, 1988, pp. 590, 567-569, 583-584.
J. Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989, pp. 17.2-17.9.
New England Nuclear Catalog, 1983, pp. 139-140.
Beuaudi, V.P. et al. J. Infect. Dis., 158:754-760. Oct., 1988.
Hunt Jeffrey C.
Pilot-Matias Tami J.
Robinson John M.
Abbott Laboratories
Becker Cheryl L.
Housel James C.
Swartz Rodney P.
Weinstein David L.
LandOfFree
Borrelia burgdorferi antigens and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Borrelia burgdorferi antigens and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Borrelia burgdorferi antigens and uses thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-595807