Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1994-01-25
1996-10-08
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 800DIG43, 4351723, 435 691, 435 701, 435 72, 435190, 435193, 47 58, A01H 500, A01H 100, C12P 2102, C12P 1902
Patent
active
055633242
ABSTRACT:
Transgenic plants have been produced which have been engineered to produce physiologically significant levels of sugar alcohols, or polyols, which is not natively produced by plants of the species. Transgenic plants have been engineered to express a bacterial mannitol-1-P dehydrogenase which, in the reverse reaction in the plant cells, produces mannitol from fructose in a plant which does not natively produce mannitol. Levels of polyols in plant cells have been associated with osmotic regulation and thereby with water stress tolerance. The transgenic plants have significant research value, and, surprisingly, seem to exhibit enhanced growth rates and vigor, and stress tolerance. Another polyol-producing enzyme gene has been isolated from a stress tolerant plant.
REFERENCES:
patent: 4771002 (1988-09-01), Gelvin
F. Koller et al. Hoppe-Seyler's Z. Physiol. Chem., vol. 357 (Nov. 1976) pp. 465-1468.
A. Fersht, Enzyme Structure & Mechanism, Reading & San Francisco, W. H. Freeman & Co., pp. 256-257 (1977).
P. Dittrich et al., Phytochemistry, vol. 26, #7 (1987) pp. 1925-1926.
R. Aebersold et al. PNAS, vol. 84 (Oct. 1987) pp. 6970-6974.
Sambrook, J., et al., "Molecular Cloning, A Laboratory Manual", Cold Spring Harbor Lab Press, Cold Spring Harbor, NY (1989), pp. 11.2-11.9, 11.45-11.49 , & 11.52-11.61.
An, G., et al., "Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System, " 81 Plant Physiol. 301-305 (1986).
Benfey, P. N. and Chua, N. H., "The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants," 250 Science 959-966 (1990).
Bevan, M., "Binary Agrobacterium Vectors for Plant Transformation," 12 Nucleic Acid Res. 8710-8721 (1984).
Bieleski, R. L., "Sugar Alcohols," in 13A Encycl. Plant Physiol., 158-192 (1986).
Davis, T., et al., "Nucleotide Sequence of the Mannitol (mtl) Operon in Escherichia coli," 2 Molecul. Microbiol. 405-412 (1988). Gives an incorrect sequence for mtl gene.
Fellman and W. H. Loescher, "Comparative Studies of Sucrose and Mannitol Utilization in Celery" (Apium graveolens) (1987).
Fox, T. C., et al., "Developmental Changes in Photosynthetic Gas Exchange in the Polyol-Synthesizing Species, Apium graveolens L., (Celery)," 82 Plant Physiol. 307-31 (1986).
Fromm, M., et al., "Expression of Genes Transferred into Monocot and Dicot Plant Cells by Electroporation," 82 PNAS 5824-5828 (1985).
Fromm, M. E., et al., "Stable Transformation of Maize After Gene Transfer by Electroporation," 319 Nature 791-793 (1986).
Guerineau, F., et al., "An Expression Cassette for Targeting Foreign Proteins into Chloroplasts," 16 N.A.R. 11380 (1980).
Harkins, K. R., et al., "Expression of Photosynthesis-Related Gene Fusions is Restricted by Cell Type in Transgenic Plants and Transfected Protoplasts," 87 PNAS 816-820 (1990).
Horsch, R. B., et al., "A Simple and General Method for Transferring Genes into Plants," 227 Science 1229-1231 (1985).
Ikawa, T., et al., "Enzymes Involved in the Last Steps of the Biosynthesis of Mannitol in Brown Algae," 13 Plant Cell Physiol. 1017-1029 (1972).
Jefferson, R. A., et al., "GUS Fusions: .beta.-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higer Plants," 6 EMBO J. 3901-3911 (1987).
Jennings, D. H., "Polyol Metabolism in Fungi," 25 Adv. Microbial. Physiol. 149-193 (1984).
Jiang, W. and W. G. Niehaus, "Cloning and Sequencing of mtl D Gene from Aspergillus parasiticus," 107 J.C.B. 746A (1988) (abstract).
Jiang, W., et al., "The Corrected Sequence of the Mannitol (mtl) Operon in Escherichia coli," 4 Molecul. Microbiol. 2003-2006 (1990).
Lam, E. and Chua, N-H, "GT-1 Binding Site Confers Light Responsive Expression in Transgenic Tobacco," 248 Science 471-474 (1990).
Lee, C. A. and M. H. Saier, Jr., "Use of Cloned mtl Genes of Escherichia coli to Introduce mtl Deletion Mutations into the Chromosome," 153 J. Bacteriology 685-692 (1983).
Lee, Y. C., "High-Performance Anion--Exchange Chromatography for Carbohydrate Analysis," 189 Anal. Biochem. 152-162 (1990).
Lewis, D. H., "Physiology and Metabolism of Alditols," In: Storage Carbohydrates in Vascular Plants, Ed by D. H. Lewis, Cambridge University Press, Cambridge, pp. 151-179 (1984).
Loescher, W. H., et al., "Other Carbohydrates as Translocated Carbon Sources: Acyclic Polyols and Photosynthetic Carbon Metabolism," In: Regulation of Carbon Partitioning in Photosynthetic Tissue. Ed by Heath, R. L. Priess J. Am. Soc. Plant Physiol., Rockville, MD pp. 309-332 (1985).
Meinzer, F. C., et al., "Leaf Water Relations and Maintenance of Gas Exchange in Coffee Cultivars Grown in Drying Soil," 94 Plant Physiol. 1781-1787 (1990).
Napoli, C., et al., "Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans," 2 Plant Cell 279-289 (1990).
Paul, M. J. and Cockburn, W., "Pinitol, a Compatible Solute in Mesembryanthemum Crystallinum L.?," 40 J. Exper. Bot. 1093-1098 (1989).
Peacock, J., et al., "Development and Environmental Regulation of the Maize Alcohol Dehydrogenase l (Adhl) Gene: Promoter-Enhancer Interactions" In: Plant Gene Systems and Their Biology, Ed Key J., McIntosh, L. AR Liss, New York, pp. 263-277 (1987).
Popp, M., "Chemical Composition of Australian Mangroves. II. Low Molecular Weight Carbohydrates," 113 Z. Pflanzenphysiol. 411-421 (1984).
Powlsen, C. and Chua N-H, "Dissection of 5' Upstream Sequences for Selective Expression of the Nicotiana plumbaginifolia rbcS-8B Gene." 214 Mol. Gen. Genet. 16-23 (1988).
Rocklin, R. D. and Pohl, C. A., "Determination of Carbohydrates by Anion Exchange Chromatography with Pulsed Amperometric Detection," 6 J. Lig. Chromat. 1577-1590 (1983).
Rumpho, M. E., et al., "A Pathway for Photosynthetic Carbon Flow to Mannitol in Celery Leaves," 73 Plant Physiol. 869-873 (1983).
Sharkey, T. D., "Photosynthesis in Intact Leaves of C.sub.3 Plants: Physics, Physiology and Rate Limitations," 51 Bot. Rev. 53-105 (1985).
Thompson, M. R., et al., "Mannitol Metabolism in Cultured Plant Cells," 67 Physiol. Plant 365-369 (1986).
Trip, P., et al., "Metabolism of Mannitol in Higher Plants," 51 Am. J. Bot. 828-835 (1964).
Tyree, M. T., "The Relationship Between the Bulk Modules of Elasticity of a Complex Tissue and the Mean Modules of its Cells." Ann. Bot. 47:547-559 (1981).
van der Krol, A. R., et al., "Flavonoid Genes in Petunia: Addition of a Limited Number of Genes Copies May Lead to a Suppression of Gene Expression," 2 Plant Cell 291-299 (1990).
von Schaewen, A., et al., "Expression of a Yeast-Derived Invertase in the Cell Wall of Tobacco and Arabidopsis Plants Leads to Accumulation of Carbohydrate and Inhibition of Photosynthesis and Strongly Influences Growth and Phenotype of Transgenic Tobacco Plants," 9 EMBO Journal 3033-3044 (1990).
Wyn Jones, R. G., et al., "A Hypothesis on Cytoplasmic Osmoregulation" In: Regulation of Cell Membrane Activities in Plants. Ed. Marre E. Elsevier/North Holland Biomed Press, Amsterdam. pp. 121-136 (1997).
Lewis, D. H. and D. C. Smith, "Sugar Alcohols (Polyols) in Fungi and Green Plants, I. Distribution, Physiology and Metabolism," 66 New Phytol. 143-184 (1967).
Cushman, J. C., et al., "Salt Stress Leads to Differential Expression of Two Isogenes of Phosphoenolpyruvate Carboxylase during Crassulacean Acid Metabolism Induction in the Common Ice Pland", 1 Plant Cell 715-725 (1989).
Sivak, M. N., "Increasing Photosynthesis by Genetic Manipulation: Difficulties and Prospects," In: Enzymatic and Model Carboxylation and Reduction Reactions for Carbon Dioxide Utilization, Ed. by M. Aresta and J. V. Schloss, Kluwer Academic Publishers, Netherlands. pp. 301-320 (1990).
Tarczynski, et al., "Expression of a Bacterial mtlD gene in Transgeric Tobacco Leads to Production and Accumulation of Mannitol," 89 Proc. Natl. Acad. Sci. (1992) (print draft).
Vernon D. M. and H. J. Bohnert, "A Novel Methyl Transferase Inducted by Osmotic Stress in the Faculative Halophyte Mesembryanthemum crystalliunm," EMBO (Manuscript).
Tarczynski, et al., "Stress Protection of Transgenic Tobacco by Production of the Osmolyte Mannitol," Sci
Bohnert Hans J.
Jensen Richard G.
Tarczynski Mitchell C.
Vernon Daniel M.
Fox David T.
Rories Charles
The Arizona Board of Regents on behalf of the University of Ariz
LandOfFree
Transgenic plants with altered polyol content does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Transgenic plants with altered polyol content, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transgenic plants with altered polyol content will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-58795