Novel erythropoietin product and method for the preparation ther

Details Novel erythropoietin product and method for the preparation ther Novel erythropoietin product and method for the preparation ther

1982-03-03

1983-08-09

Rosen, Sam

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424177, A61K 3522

Patent

active

043978401

ABSTRACT:
The invention provides a novel erythropoietin product prepared from the urine of healthy human but exhibiting no inhibitory effect against erythropoiesis as is the most disadvantageous problem in the product prepared from the urine of healthy human. The method for the preparation of such a product comprises adsorbing the crude erythropoietin product obtained from the urine of healthy human on to a weakly basic anion exchanger from a neutral or weakly acidic aqueous solution containing 0.1 to 0.2 mole per liter of an inorganic neutral salt such as sodium chloride and then eluting the thus adsorbed ingredients with an aqueous eluant solution containing from 0.5 to 0.7 mole per liter of an inorganic neutral salt.

REFERENCES:
patent: 4303650 (1981-12-01), Takezawa et al.
Miyake et al.-J. Biol. Chem., vol. 252, No. 15, (Aug.10, 1977), pp. 5558-5564.

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