Multilayer analytical element for analysis of cholesterol

Details Multilayer analytical element for analysis of cholesterol Multilayer analytical element for analysis of cholesterol

1986-07-08

1989-05-02

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435 25, 435 28, C12Q 160

Patent

active

048267619

ABSTRACT:
An integral multilayer analytical element for analysis of cholesterol which comprises a water-impermeable light-transmissive support, a hydrophilic layer and a spreading layer superposed in this order, and contains cholesterol esterase, cholesterol oxidase, peroxidase and a coloring reagent composition, which is characterized, in that the above coloring reagent composition is a combination of 4-aminoantipyrine or its derivative and 2-hydroxy-3,5-dichlorobenzene sulfonic acid, and in that pH of the layer where coloring reaction proceeds is kept in the range of 7.5 to 9.
This analytical element is stable, and capable of preserving more than 8 months without fogging.

REFERENCES:
patent: 3983005 (1976-09-01), Goodhue et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4089747 (1978-05-01), Jungfleisch
patent: 4291121 (1981-09-01), Acquati et al.
patent: 4414326 (1983-11-01), Goldberg

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