Purified luciferase from luciola lateralis

Details Purified luciferase from luciola lateralis Purified luciferase from luciola lateralis

1991-08-29

1994-10-04

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435243, 435815, 435816, C12N 902, C12N 100

Patent

active

053525981

ABSTRACT:
A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

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