DNA analyzing method and device therefor

Details DNA analyzing method and device therefor DNA analyzing method and device therefor

Filed on

1995-11-09

Date issued

2000-08-22

Examiner

Horlick, Kenneth R.

Patent Class

Chemical apparatus and process disinfecting, deodorizing, preser

SubClass

Analyzer, structured indicator, or manipulative laboratory...

Other Related Categories

435 6, 435 911, 4352872, 436800, 436807, 436808, 536 231, 536 243, 536 2433, 935 77, 935 78, 935 86, 935 87, 359350, 422 8205, 422 8208, G01N 2129

Type

Patent

Status

active

Patent number

061067776

Description

ABSTRACT:
The object of the present invention is to provide a method capable of analyzing the presence or absence of a target DNA sequence, the level and sequence characteristics thereof at a high sensitivity, and a device therefor, wherein the overall process from pretreatment to the recovery of DNA information and the analysis thereof can be completed in a speedy fashion by the simple device structure and procedures.
Therefore, by preparing a single-stranded DNA fragment of a target DNA region, detecting the change in the absorbance of the single-stranded DNA sample while changing the denaturing condition of the conformation of the single-stranded DNA fragment by a denaturing condition regulatory means, and analyzing the curve of the change in the absorbance over the modification in the denaturing condition, the sequence information of the single-stranded DNA, namely the target DNA, can be generated in a rapid and simple manner.

REFERENCES:
patent: 4801550 (1989-01-01), Kapuscinski et al.
patent: 5200313 (1993-04-01), Carrico
patent: 5591582 (1997-01-01), Bos et al.
Razlutskii et al., "The effect of nucleotide substitution on DNA denaturation profiles," Nucleic Acids Research, vol. 15, No. 16, pp. 6664-6676, 1986.
Inoki et al., "In situ microspectrofluorometry of nuclear and kinetoplast DNA in Trypanosoma gambiense," Zentralbl. Bakteriol., Parasitenkd., Infektionskr. Hyg., Abt. 1: Orig., Reihe A (1979), 244(2-3), 327-30; STN, File Chemical Abstracts, Abstract No. 9.
Rapid And Sensitive Detection of Point Mutations and DNA Polymorphisms Using the Polymerase Chain Reaction; Masato Orita, Youichi Suzuki, Takao Sekiya and Kenshi Hayashi, Genomics 5, pp. 874-879 (1989).
Thermodynamics of RNA Folding in a Conserved Ribosomal RNA Domain, Lance G. Laing and David E. Draper, Academic Press Limited 1994, pp. 560-576.
Nomenclature For Factors of the HLA System, Immunogenetics, 1990, pp. 131-140.
The Effect of Nucleotide Substitution on DNA Denaturation Profiles, I.V. Razlutskii, L. S. Shlyakhtenko and Yu. L. L. Lyubchenko, Nucleic Acids Research, vol. 15, No. 16, 1987, pp. 6664-6677.
Generation of Single-Stranded DNA by the Polymerase Chain Reaction and its Application to Direct Sequencing of the HLA-DQA Locus, U. Gyllensten & H. Erlich, Pro. Natl. Acad. Sci. USA, vol. 85, pp. 7652-7656, Oct. 1988.
Comparative Studies on the Secondary Structure of Ovalbumin Messenger RNA and Its Complementary DNA Transcript; Nguyen T. Van et al.; Biochemistry, vol. 16, No. 18, 1977, pp. 4090-4100.
F-SSCP: Fluorescence-Based Polymerase Chain Reaction-Single-strand Conformation Polymorphism (PCR-SSCP) Analysis; Reiko Makino et al.; PCR Methods and Applications, Aug. 1992, vol. 2, No. 1, pp. 10-13.
Detecting Single-Base Mutations, Jane Prosser, Tibtech, Jun. 1993, vol. 11, pp. 238-246.

Affiliated with

Also associated with

Rating

DNA analyzing method and device therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA analyzing method and device therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA analyzing method and device therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-577139