Immunoassay by light scattering spectroscopy

Details Immunoassay by light scattering spectroscopy Immunoassay by light scattering spectroscopy

1976-03-01

1978-03-21

Shapiro, Lionel M.

Textiles: fiber preparation

1951035R, 1951035V, 356103, 424 12, G01N 3100

Patent

active

040802647

ABSTRACT:
This inventin provides a means for determining the concentration of any of a wide range of antibody or antigen molecules with a high degree of specificity, accuracy and sensitivity. Antigen or antibody concentration is determined by effecting an agglutination reaction in a liquid medium and determining the mean diffusion constant of the agglutinated reaction product by quasi-elastic light scattering spectroscopy. The measured mean diffusion constant then is compared with a standard quantitative relationship between mean diffusion constant and concentration of the antigen or antibody being tested. By this means one may specifically ascertain the absolute concentration of the antigen or antibody in question in the sample being analyzed. In addition to detecting antigen or antibody molecules, the process of this invention can be used to determine the concentration of any substance capable of specifically promoting or inhibiting an agglutination reaction such as viruses, white blood cells or the like.

REFERENCES:
patent: 3905767 (1975-09-01), Morris et al.
patent: 3967901 (1976-07-01), Rodriguez
patent: 3984533 (1976-10-01), Uzgiris
patent: 3990851 (1976-11-01), Gross et al.

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