Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1997-05-23
2000-07-11
Hobbs, Lisa J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435100, 4352523, 435440, 536 232, 536 243, C12N 924, C12N 120, C12P 1912, C07H 2104
Patent
active
060871466
ABSTRACT:
Disclosed are a recombinant thermostable enzyme, which converts maltose into trehalose and is stable up to a temperature of about 80.degree. C. even when incubated at pH 7.0 for 60 min, a preparation of the enzyme, a DNA encoding the enzyme, a recombinant DNA containing the DNA, a transformant, and an enzymatic conversion method of maltose by using the enzyme.
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Nishimoto, et al, "Existence of a Novel Enzyme Converting Maltose into Trehalose"; Biosci, Biotech, Biochem, 59(11):2189-2190 (1995).
Nishimoto, et al, "Purification and Properties of a Novel Enzyme, Trehalose Synthase, from Primelobacter sp. R48"; Biosci, Biotech, Biochem, 60(4):640-644 (1996).
Nishimoto, et al, "Purification and Characterization of a Thermostable Trehalose Synthase from Thermus aquaticus"; Biosci. Biochem. Biotech., 60(5):835-839 (1996).
Sambrook, et al, Molecular Cloning A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press (Plainview, NY 1989); pp. xi=xxxviii.
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Southern, E.M., "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis"; J. Mol. Biol., 98:503-517 (1975).
Tsusaki, et al, "Cloning and sequencing of trehalose synthase gene from Primelobacter sp. R48"; Biochimica et Biophysica Acta, 1290:1-3 (1996).
Kubota Michio
Tsusaki Keiji
Hobbs Lisa J.
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
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