Nucleic acid hybridization assay employing detectable anti-hybri

Details Nucleic acid hybridization assay employing detectable anti-hybri Nucleic acid hybridization assay employing detectable anti-hybri

1988-04-25

1993-04-06

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435803, 435810, 436501, 436508, 436512, 436808, 536 2432, 5303871, 935 2, 935 78, C12Q 168

Patent

active

052003132

ABSTRACT:
A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.

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