Nucleic acid probes and amplification oligonucleotides for Neiss

Details Nucleic acid probes and amplification oligonucleotides for Neiss Nucleic acid probes and amplification oligonucleotides for Neiss

1995-06-07

1998-05-05

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435912, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

057472521

ABSTRACT:
The present invention discloses hybridization assay probes, amplification primers, nucleic acid compositions and methods useful for detecting Neisseria nucleic acids. Hybridization assay probes and amplification primers that selectively detect Neisseria meningitidis and distinguish those Neisseria meningitidis from Neisseria gonorrohoeae are disclosed. Other hybridization probes selectively detect Neisseria gonorrohoeae and not Neisseria meningitidis are also described.

REFERENCES:
patent: 5030557 (1991-07-01), Hogan et al.
Radstrom et al, "Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococci using a seminested PCR strategy", J. Clin. Microbiol. 32(11):2738-2744, Nov. 1994.
Muralidhar et al, "Design and characterization of PCR primers for detection of pathogenic Neisseriae", Mol. Cell. Probes 8:55-61, Feb. 1994.
Greisen et al, "PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in the cerebrospinal fluid", J. Clin. Microbiol. 32(2):335-351, Feb. 1994.
Linz et al, "Systematic studies on parameters influencing the performance of the polymerase chain reaction", J. Clin. Chem. Clin Biochem. 28:5-13, 1990.

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