Method for cloning and producing the NspI restriction endonuclea

Details Method for cloning and producing the NspI restriction endonuclea Method for cloning and producing the NspI restriction endonuclea

Filed on

1998-08-18

Date issued

2000-02-22

Examiner

Patterson, Jr., Charles L.

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Enzyme , proenzyme; compositions thereof; process for...

SubSubClass

Hydrolase

Other Related Categories

4352523, 4353201, 536 232, C12N 922, C12N 1555

Type

Patent

Status

active

Patent number

06027929&

Description

ABSTRACT:
The present invention relates to clones which express the recombinant NspI restriction endonuclease using a NspI methylase premodified E. coli K strain RR1 (.lambda.DE3) and to methods for producing and purifying said enzyme.

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Szomolanyi et al., Gene 10:219-225 (1980).
Janulatis et al., Gene 20:197-204 (1982).
Kiss et al., Gene 21:111-119 (1983).
Walder et al., J. Biol. Chem. 258:1235-1241 (1983).
Fomenkov et al., Nucl. Acids Res. 22:2399-2403 (1994).
Skoglund et al., Gene 88:1-5 (1990).
Xu, S-Y., et al. (1998) 260, 226-231.

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