Packaging system for extended shelf life of microbial system

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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4353071, 4353091, 435810, C12M 124

Patent

active

057337772

ABSTRACT:
A packaging system is disclosed for use in storing and conveniently and sterilely activating a useful microbial system in a culture medium. The system includes a conveniently sized bottle of sterile culture broth in a bottle capped with a conventional screw on cap. A second storage cap is provided having an elastomeric o-ring seal at its top and an impermeable sealing disc across the o-ring seal. The space inside the o-ring seal between the sealing disc and the top of the cap contains the lyophilized microorganism inoculant. Removal of the sealing disc and replacement of the first cap with the second cap on the bottle of culture broth then, upon inversion, inoculates the culture broth.

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patent: 4009285 (1977-02-01), Spooner
patent: 4392492 (1983-07-01), Pick
patent: 4589835 (1986-05-01), St. Amand
patent: 5155039 (1992-10-01), Chrisope et al.

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