Method for quantitative measurement of trace enzyme and substrat

Details Method for quantitative measurement of trace enzyme and substrat Method for quantitative measurement of trace enzyme and substrat

1991-07-08

1994-04-05

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 74, 435 75, 435 14, 435 23, 436170, 422 56, C12Q 100, G01N 3353

Patent

active

053004213

ABSTRACT:
A synthetic substrate used for quantitative analysis of a trace enzyme. The substrate has a molecular structure (B)-(A)-(C) comprising at least one structure (A) catalytically affected by the enzyme to be analysed, at least one photographically active labelling structure (B) linked to the structure (A) and at least one specific adsorbing structure (C) linked to the structure (A). The substrate is used in a quantitative analysis of a trace enzyme, in which the structure (A) or the linkage between the structure (A) and the structure (C), or the linkage between the structure (B) and the structure (A) is cleaved by the action of the analyte enzyme. The reaction product of the enzymatic reaction is separated by allowing the same to contact with an adsorbent for the structure (C). The separated reaction product is developed photographically and then the optical density of the resultant developed silver and/or colored dye is measured.

REFERENCES:
patent: 4414323 (1983-11-01), Masuda et al.
patent: 4414325 (1983-11-01), Masuda et al.
patent: 4966607 (1990-10-01), Shinoki et al.
Pharmacia Fine Chemicals (1983) Affinity Chromatography, p. 4.

Affiliated with

Also associated with

Rating

Method for quantitative measurement of trace enzyme and substrat does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for quantitative measurement of trace enzyme and substrat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for quantitative measurement of trace enzyme and substrat will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-510341