Methods and kits for performing nucleic acid hybridization assay

Details Methods and kits for performing nucleic acid hybridization assay Methods and kits for performing nucleic acid hybridization assay

1985-06-13

1988-06-14

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 435814, 436501, 436527, 436531, 436808, 436824, 935 2, 935 3, 935 6, 935 78, C12Q 168

Patent

active

047511770

ABSTRACT:
A method for the isolation and quantitative detection of a selected single-stranded target polynucleotide from solution. The target polynucleotide is hybridized in solution to a single-stranded mediator polynucleotide, a probe polynucleotide, and an immobilized polynucleotide sequence. The sequence of the mediator polynucleotide comprises a first sequence complementary to a first portion of the target polynucleotide sequence and a second nucleotide sequence complementary to a portion of a single-stranded immobilized polynucleotide sequence. The probe polynucleotide, which carries a detectable label, is complementary to a second portion of the necleotide sequence of the target. The immobilized polynucleotide is immobilized by attachment to a solid support, and, through hybridization to the mediator polynucleotide, functions to immobilize the entire immobilized polynucleotide/target polynucleotide/probe polynucleotide "sandwich".

REFERENCES:
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patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4522922 (1985-06-01), Carro et al.
Bornkamm et al., Curr. Top. Microbiol. Immunol., 104, 288-298, (1983).
Dunn et al., Cell, 12, 23-36, (1977).
Ranki et al., Curr. Top. Microbiol. Immunol., 104, 307-318, (1983).
Ranki et al., Gene, 21, 77-85, (1983).
Wu et al., Proc. Natl. Acad. Sci., (USA), 78, 7059-7063, (1981).

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