DNA sequence, related probes and primers for the detection of St

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 536 231, 536 243, 536 2432, C12Q 168, C12P 1934, C07H 2100, C07H 2102

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active

060047542

ABSTRACT:
Disclosed herein is newly-identified DNA sequence from Streptococcus agalactiae ("GBS") designated GBS3.1. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the species-specific detection of S. agalactiae. Preferably, S. agalactiae is detected by amplifying the S. agalactiae nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, S. agalactiae nucleic acids are amplified and detected by thermophilic strand displacement amplification (tSDA).

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Chatellier, Sonia et al., Characterization of Streptococcus agalactiaeStrains by Randomly Amplified Polymorphic DNA Analysis; J. of Clin. Microbiol.; 35, pp. 2573-2579 (1997).
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