Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1980-09-05
1983-02-08
Sebastian, Leland A.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
A61K 4300
Patent
active
043729410
ABSTRACT:
A rapid, efficient and accurate method is provided for quantitatively assaying for an enzyme, such as angiotensin converting enzyme, an aminopeptidase, a carboxypeptidase, or trypsin, which catalyzes hydrolysis of a radiolabelled substrate to produce a radiolabelled remnant product preferentially soluble in a water-immiscible organic solvent, whereby the assay is conducted in a vial having a volume of not more than 20 milliliters, preferably 7 ml. or less, by incubating substrate in buffer and sample, stopping the reaction after a predetermined time with, e.g., acid or base, adding the water-immiscible organic solvent containing scintillant, capping the vial, mixing by inverting the vial whereupon a spontaneous phase separation occurs. The radioactivity of the organic phase is then counted in an appropriate scintillation counter and the enzymic activity is thereupon calculated.
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