Stable DNA constructs for expression of .alpha.-1 antitrypsin

Details Stable DNA constructs for expression of .alpha.-1 antitrypsin Stable DNA constructs for expression of .alpha.-1 antitrypsin

1984-10-22

1990-06-05

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435255, 435256, 435320, 536 27, 935 28, 935 37, 935 69, C12P 2100, C12P 2102, C12N 1500, C12N 700

Patent

active

049313738

ABSTRACT:
Methods are provided for producing .alpha.-1-antitrypsin in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selectable marker whereby the growth conditions for selection may comprise a conventional complex medium.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4599311 (1986-07-01), Kawasaki
Bollen et al., 1983, "Cloning and Expression in E. coli of Full-Length Complementary DNA Coding for Human .alpha.-Antitrypsin", DNA vol. 2(4) 255-64.
Walsh et al., 1983, "Cloning of Genes that Complement Yeast Hexokinase & Glucokinase Mutants", J. Bact., vol. 154, 1002-1004.
Carter et al., 1983, "Genetic Control of Cell Proliferation", in Yeast Genetics, Spencer et al. (Ed.), Springer-Verlag, pp. 1-28.
Nasmyth et al., 1980, "Isolation of Genes by Complementation in Yeast: Molecular Cloning of a Cell-Cycle Gene", Proc. Natl. Acad. Sci., vol. 77, 2119-2123.
Kuruchi et al., Proc. Natl. Acad. Sci., vol. 78, pp. 6826-6830, 1981, "Cloning of Sequence of cDNA coding for .alpha.-1-Antitrypsin".
Chandra et al., Biochem. Biophys. Res. Comm., vol. 103, pp. 751-758, 1981.
Strathern et al., Mol. Biol. of the Yeast Saccharomyces Life Cycle and Inheritance, pp. 97-142, 1981.
Strathern et al., Mol. Biol. of the Yeast Saccharomyces Metabolism and Gene Expression, 1982, pp. 640-651.
Kuo et al., 1983, "Cloning of Saccharomyces cerevisiae DNA Replication Gene: Isolation of the CDC 8 Gene . . . ", Mol. Cell Biol., vol. 3, 1730-37.
Alber et al., 1982, "Nucleotide Sequence of the Triose Phosphate Isomerase Gene of S. cerevisiae", J. Mol. App. Gen., vol. 1, 419-34.
Arendes et al., 1983, "Yeast 2-.mu.m Plasmid DNA Replication in vitro: Purification of the CDC8 Gene Product . . . ", Proc. Natl. Acad. Sci., vol. 80, 673-77.
Owen et al., The New England Journal of Medicine (1983), pp. 694-698, "Mutation of Antitrypsin to Antithrombin".
Carrell et al., Nature, 298 (1982), pp. 329-334, "Structure and Variation of Human .alpha.-1-Antitrypsin".

Affiliated with

Also associated with

Rating

Stable DNA constructs for expression of .alpha.-1 antitrypsin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Stable DNA constructs for expression of .alpha.-1 antitrypsin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable DNA constructs for expression of .alpha.-1 antitrypsin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-491282