Method, reagents and apparatus for the rapid identification of N

Details Method, reagents and apparatus for the rapid identification of N Method, reagents and apparatus for the rapid identification of N

1978-08-23

1980-06-17

Jones, Raymond N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435300, 435871, C12Q 104

Patent

active

042084806

ABSTRACT:
The disclosure is directed to a method, reagents and apparatus for the rapid (one to four hours) identification of Neisseria gonorrhoeae and Neisseria meningitidis from cultures grown on a selective or a non-selective medium. One drop of growth suspended in sterile salt solution is placed in each of a plurality of miniature reaction chambers supported on a common base and incubated at 35.degree.-37.degree. C. for one to four hours. A dried substrate and buffer is contained in each chamber. A detector reagent such as a diazo dye in an aqueous solution with a polar solvent is added to each chamber after incubation. The color change in each chamber is noted and compared with a profile to identify N. gonorrhoeae and Neisseria meningitidis. Synthetic substrates rather than sugars are used in the reaction chambers. The substrates are naphthyl derivatives, .beta.-naphthyl or .beta.-naphthylamides, and are

REFERENCES:
patent: 3830703 (1974-08-01), Beckford
patent: 3832288 (1974-08-01), Rollender et al.
patent: 3957584 (1976-05-01), Kronish et al.
patent: 4026767 (1977-05-01), Shih et al.
patent: 4039387 (1977-08-01), Simpson et al.
patent: 4048016 (1977-09-01), Otto

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