Electricity: measuring and testing – Particle precession resonance – Using a nuclear resonance spectrometer system
Patent
1998-10-01
2000-10-17
Oda, Christine K.
Electricity: measuring and testing
Particle precession resonance
Using a nuclear resonance spectrometer system
324309, G01V 300
Patent
active
061337366
ABSTRACT:
A method for obtaining a nuclear magnetic resonance (NMR) correlation spectrum of heteronuclear spin systems, in particular comprising large molecules, especially biological macromolecules in solution, the spin system being subjected to a homogeneous magnetic field B.sub.0, being irradiated by a sequence of radio frequency (rf) pulses, is characterized in that the spin system comprises at least two kinds of spin 1/2 nuclei I and S being coupled to each other, whereby the sequence of rf pulses is chosen such that line broadening in the observed spectrum due to transverse relaxation (T.sub.2) is significantly reduced because of cross correlation between dipole--dipole (DD) coupling of the spins and chemical shift anisotropy (CSA), giving rise to different relaxation rates of the individual multiplet components of the spin system and chosen such that the relaxation effects of the two different mechanisms cancel each other out to a large degree. Thus, even very large biological macromolecules can be measured.
REFERENCES:
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L.E. Kay et al.: "Pulse Sequences for Removal of the Effects of Cross Correlation between Dipolar and Chemical-Shift Anisotropy Relaxation Mechanisms ont eh Measurement of Heteronuclear T1 and T2 Values in Proteins", Journal of Magnetic Resonance, vol. 97, 1992, pp. 359-375.
J.Boyd et al: "Influence of cross-correlation between dipolar and chemical shift anisotropy relaxation mechanisms upon the transverse relaxation rates of 15-N in macromolecules", Chemical Physics Letters, vol. 187, No. 3, Dec. 6, 1991, pp. 317-324.
S. Wimperis et al.: "Relaxation-allowed cross-peaks in two-dimensional N.M.R. correlation spectroscopy", Molecular Physics, vol. 66, No. 5, 1989, pp. 897-919.
G. Bodenhausen et al.: "Natural Abundance Nitrogen-15 NMR by Enhanced Heteronuclear Spectroscpy", Chemical Physics Letters, vol. 69, No. 1, Jan. 1980, pp. 185-189.
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Pervushin Konstantin
Riek Roland
Wider Gerhard
Wuthrich Kurt
Bruker AG
Oda Christine K.
Shrivastav Brij B.
Vincent Paul
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