Ligation assembly and detection of polynucleotides on solid-supp

Details Ligation assembly and detection of polynucleotides on solid-supp Ligation assembly and detection of polynucleotides on solid-supp

1998-11-12

1999-08-24

Marschel, Ardin H.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 912, 436501, 935 77, 935 78, C07H 2100

Patent

active

059426098

ABSTRACT:
The present invention concerns methods of assembly of a polynucleotide on a solid-support by performing steps of annealing, ligation, and extension. The steps may be repeated in a cyclical manner to assemble immobilized double- or single-stranded polynucleotides with functional gene properties. The immobilized polynucleotides may be amplified by the polymerase chain reaction, and detected and quantitated by an exonuclease assay with a self-quenching, fluorescent probe. The polynucleotide may be cleaved from the solid-support by chemical or enzymatic cleavage.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5093251 (1992-03-01), Richards et al.
patent: 5158877 (1992-10-01), Edwards et al.
patent: 5470724 (1995-11-01), Ahern
patent: 5503995 (1996-04-01), Khudyakov et al.

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