Attenuated strain of Leishmania

Details Attenuated strain of Leishmania Attenuated strain of Leishmania

1995-06-02

2000-10-17

Navarro, Mark

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Protozoa, media therefor

4242691, C12N 110

Patent

active

061330175

ABSTRACT:
Differentially expressed Leishmania genes and proteins are described. One differentially expressed gene (A2) is expressed at significantly elevated levels (more than about 10 fold higher) in the amastigote stage of the life cycle when the Leishmania organism is present in macrophages than in the free promastigote stage. The A2 gene encodes a 22 kD protein (A2 protein) that is recognized by kala-azar convalescent serum and has amino acid sequence homology with an S-antigen of Plasmodium falcilparum Vietnamese isolate VI. Differentially expressed Leishmania genes and proteins have utility as vaccines, diagnostic reagents, as tools for the generation of immunological reagents and the generation of attenuated variants of Leishmania.

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