Production of soluble recombinant proteins

Details Production of soluble recombinant proteins Production of soluble recombinant proteins

1988-03-17

1990-08-14

Martinell, James

Chemistry: analytical and immunological testing

Blood gas

4351721, 4351723, 4352523, 435320, 536 27, 935 11, 935 46, 935 72, C12P 2100, C12N 1500, C12N 120, C07H 2104

Patent

active

049487299

ABSTRACT:
A system for expression of coding sequences for desired heterologous proteins in procaryotic hosts whereby the protein is produced intracellularly in soluble, biologically active form, is disclosed. The expression is obtained by ligation of the coding sequence downstream of, and proximally to, but out of reading frame with, the terminated leader encoding sequence for a secreted bacterial protein, such as alkaline phosphatase. The resultant proteins are influenced by the leader sequence codons to effect the desired three-dimensional conformation, but not to effect secretion.

REFERENCES:
Michaelis et al., "In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli", J. Bacteriol. 154:356.
Roberts et al., "A general method for maximizing the expression of a cloned gene", Proc. Natl. Acad. Sci. USA 76:760 (1979).
Genes, Lewin, 1983, John Wiley & Sons, New York, pp. 679 and 683.
Normark et al., Ann. Rev. Genet., 17:499-525 (1983).

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