Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1988-08-18
1990-11-20
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435944, C12P 740, C12R 188
Patent
active
049719076
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
This invention relates to a method for producing pyruvic acid by fermentation.
DESCRIPTION OF THE PRIOR ART
It has heretofore been known to use a microorganism of genus Torulopsis for production of pyruvic acid by fermentation (Journal of Japan Agricultural Chemical Society, Vol. 32, page 573; Japanese Unexamined Patent Publication No.14789/1987).
However, such method is low in accumulation as well as yield of pyruvic acid and is not industrially applicable. According to the Journal of Japan Agricultural Chemical Society Vol. 32, page 573, accumulation of pyruvic acid in use of Torulopsis candida is only at 0.5 g/l. Also, according to Japanese Examined Patent Publication No. 14789/1987, accumulation of pyruvic acid in use of Torulopsis etchellsii is 5.1 g/l. This Torulopsis etchellsii strain has the growth thereof accelerated by the presence of thiamine but does not require thiamine for its growth.
DISCLOSURE OF THE INVENTION
The inventors investigated into a method of producing pyruvic acid which would resolve the foregoing problems and would provide a higher productivity and found that microorganism belonging to genus Torulopsis, requiring thiamine and biotin for the growth thereof and having an ability to produce an appreciable amount of pyruvic acid.
An object of the present invention is to provide a method for producing and accumulating pyruvic acid in a satisfactory rate and yield.
Another object of the present invention is to provide a method for producing and accumulating pyruvic acid with little by-product.
Still another object of the present invention is to provide an improved method using new mutants.
These and other objects of the invention will become more apparent in the detailed description set forth in the following.
These objects are attained by a method for producing pyruvic acid by fermentation which is characterized by culturing a microorganism belonging to the genus Torulopsis, requiring thiamine and biotin for its growth and having an ability to produce pyruvic acid to produce and accumulate pyruvic acid in a culture broth then collecting pyruvic acid from the culture broth.
DESCRIPTION OF THE PREFERRED EMBODIMENTS OF THE INVENTION
The present invention will now be described in detail with reference to some embodiments.
According to the invention, microorganisms belonging to the genus Torulopsis, requiring thiamine and biotin for their growth and having an ability to produce pyruvic acid, are used.
If such characteristics are possessed, microorganisms having other auxotrophic requirements and characteristics such as drug resistance and varying enzyme activity are included in the scope of the invention.
In the invention, there may be used mutants requiring preferably arginine in addition to said requirement for thiamine and biotin for their growth. The auxotrophy for arginine referred to here is of a broad sense and includes the type of incomplete deficiency (or the so-called leaky type) and also the auxotrophy for the biosynthetic precursor of arginine.
Further, in the invention, there may be used mutants having preferably a pyruvate decarboxylase activity (hereinafter referred to as "PDC activity") lower than that of the parent strain in addition to said auxotrophy for thiamine and biotin. Reducing the PDC activity level is likely to serve for less metabolism of pyruvic acid to acetaldehyde, higher rate of conversion from glucose to pyruvic acid and thus greater accumulation of pyruvic acid
Also, in the invention, there may be used mutants preferably requiring isoleucine and valine in addition to said requirement for thiamine and biotin for their growth. The isoleucine requirement and valine requirement referred to here are of a broad sense and include the type of incomplete deficiency (or the so-called leaky type) and also the auxotrophy for the biosynthetic precursors of isoleucine and valine.
Further, in the invention, there may be used mutants having a resistance to aminooxyacetic acid in addition to said requirement for thiamine and biotin.
These
REFERENCES:
Derwent Abs. 87-060429/09 Noriinsho J62014789 Jan. 1987.
Miyata Reiko
Tsutsui Hiromi
Yonehara Tetsu
Yotsumoto Kyousuke
Lilling Herbert J.
Miller Austin R.
Toray Industries
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