System and method of fluorescence polarimetry

Optics: measuring and testing – By polarized light examination – With polariscopes

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356365, G01N 2140

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active

042036704

ABSTRACT:
A system and method for measurement of the polarization of fluorescence. A laser source provides a collimated linearly polarized beam of high intensity and small cross-section. A relatively small number of optical components are employed to minimize spurious fluorescence and spurious polarization which usually occurs from most optical materials. A photodetector provides an electrical signal in response to and representative of fluorescence polarization and is processed to directly produce first and second signals representing the difference and sum components of the polarization equation, the ratio of these components being directly provided without need for division computation.

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patent: 3710933 (1973-01-01), Fulwyler et al.
patent: 3850525 (1974-11-01), Kaye
Kay et al. "Twelve Functions in a New Digital Meter". Hewlett-Packard Journal, Mar. 1969, pp. 2-13.
Spencer et al. "Design, Construction, and Two Applications for an Automated Flow-Cell Polarization Fluorometer with Digital Read Out", Clinical Chemistry", vol. 19, No. 8, 1973, pp. 838-844.
Kelly et al. "Digital, Proton-Counting Fluorescence Polarometer", Analytical Chemistry, vol. 48, No. 6, May 1976, pp. 846-856.

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