Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1995-05-19
1997-03-11
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530350, 536 235, 536 2431, 4352523, 4353201, 435 691, 935 9, 935 27, 935 32, 935 71, 935 73, C07K 14705, C07K 1447, C12N 1586, C07H 2104
Patent
active
056102828
ABSTRACT:
The present invention relates to the molecular cloning and expression of the D.sub.1 dopamine receptor protein that is linked to the activation of adenylyl cyclase activity. By constructing cell lines that express the D.sub.1 receptor, the affinities and efficacies of agonist and antagonist drugs with the receptor can be assessed.
The present invention further relates to a recombinant DNA construct that includes a vector and a DNA fragment encoding the D.sub.1 receptor. The present invention also relates to a host cell transformed with a recombinant DNA construct, so that the DNA fragment is expressed and the D.sub.1 receptor is produced. Suitable expression systems include eukaryotic and procaryotic cells, especially mammalian cells such as rat or human.
The present invention further relates to the antibody to the D.sub.1 receptor protein. For diagnostic purposes, antibodies to this receptor can be prepared by producing all or a portion of the receptor protein and injecting these into various types of mammals. Using the resulting antibodies, expression of the D.sub.1 receptor cDNA in cells can be measured.
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Mahan Lawrence C.
McVittie Loris D.
Monsma Frederick J.
Sibley David R.
Moore William W.
The United States of America as represented by the Department of
Wax Robert A.
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