Process for determining the perkallikrein content and the partia

Details Process for determining the perkallikrein content and the partia Process for determining the perkallikrein content and the partia

1986-01-24

1988-03-22

Marcus, Michael S.

Chemistry: analytical and immunological testing

Rate of reaction determination

435 13, 436 69, G01N 2178, G01N 3386, C12Q 156

Patent

active

047328606

ABSTRACT:
The present invention provides a process for the photometric determination of prekallikrein in plasma, wherein plasma is incubated with a surface activator, the extinction obtained is measured, a chromogenic thrombin substrate is then added thereto, the optically determinable group liberated therefrom is measured at short time intervals or continuously and the gradient of the linear part of the curve obtained by plotting the time against the extinction is determined as a measure of the prekallikrein content. The partial thromboplastin time of plasma is simultaneously determined by carrying out the incubation in the presence of calcium ions and a phospholipid and measuring the time it takes the extinction to reach a predetermined level.

REFERENCES:
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