Method of treatment of RNA sample

Details Method of treatment of RNA sample Method of treatment of RNA sample

2005-07-09

2011-12-13

Lu, Frank W (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C536S023100, C536S024300

Reexamination Certificate

active

08076064

ABSTRACT:
The invention relates to methods for treating samples of RNA. In an embodiment the method includes contacting the sample of RNA with an analogous DNA set to provide a DNA/RNA duplex. The analogous DNA set includes at least one sequence analogous to a small RNA. The method includes contacting the DNA/RNA duplex with an enzyme having a DNA:RNA nuclease activity to provide a digested RNA sample. Kits in accordance with the invention are also described.

REFERENCES:
patent: 5525468 (1996-06-01), McSwiggen
patent: 5573913 (1996-11-01), Rosemeyer et al.
patent: 6613516 (2003-09-01), Christians et al.
patent: 6992180 (2006-01-01), Engelhardt et al.
patent: 7288371 (2007-10-01), Bavykin et al.
patent: 2001/0014446 (2001-08-01), Heroux et al.
patent: 2003/0190661 (2003-10-01), Gruber et al.
patent: 2004/0180362 (2004-09-01), Lazar et al.
patent: 2006/0160096 (2006-07-01), Cole et al.
patent: WO 94/12518 (1994-06-01), None
patent: WO0132672 (2001-05-01), None
patent: WO0216647 (2002-02-01), None
patent: WO2005054466 (2005-06-01), None
Zhulidov et al., Simple cDNA normalization using Kamchatks Crab duplex-specific nuclease. Nucleic Acids Research, 32, e37, 2004.
The definition of microRNAs (miRNA) from Wikipedia, the free encyclopedia. Printed on Apr. 27, 2009.
RNase H from Wikipedia, the free encyclopedia. Printed on Jan. 17, 2010.
Liu, C., et al. An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. PNAS 2004, vol. 101, No. 26, pp. 9740-9744.
Ularich Lehmann and Hans Kreipe.Real-time PCR analysis of DNA and RNA Extracted from Formalin-fixed and Praffin-Embedded Biopsies, Methods, 25, 409-418, 2001.
Richard Cosstick, et al., “Fluorescent labeling of tRNA and oligodeoxynucleotides using T4 RNA ligase”, Nucleic Acids Research, vol. 12, No. 4, 1984, pp. 1791-1810.
Jorge R. Barrio, et al., “Synthesis of Modified Nucleoside 3′, 5′-Bisphosphates and their Incorporation into Oligoribonucleotides with T4 RNA Ligase”, Modified Oligoribonucleotides, vol. 17, No. 11, 1978 pp. 2077-2081.
Wang, H., et al., “Direct and sensitive miRna profiling from low-input total RNA”, vol. 13, Nov. 14, 2006, pp. 1-9, XP002408565.
European Search Report EP 06 25 3554 dated Nov. 28, 2006.
“Kyle Cole, et al., Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes”, Nucleic Research, vol. 32, No. 11 Oxford University Press 2004; pp. 1-9.
Volker Lohman, et al., “Mutations in Hepatitis C virus RNAs Conferring Cell Culture Adaptation”, Journal of Virology, Vo. 75, No. 3, Feb. 2001, pp. 1437-1449.
Ross W. Richardson, et al., “Biotin and fluorescent labeling of RNA using T4 RNA ligase”, Nucleic Acids Research, vol. 11, No. 18, 1983, pp. 6167-6184.
Thomas E. England, et al., Enzymatic Oligoribonucleotide Synthesis with T4 RNA Ligase, American Chemical Society and reprinted by permission of the copyright owner, Reprinted from Biochemistry, 1978 pp. 2070-2076.
Thomas England, et al., “3′-Terminal Labeling of RNA with T4 RNA Ligase”, Nature, vol. 275, Oct. 1978; Department of Biochemistry, University of Illinois, pp. 560-561.
Lance A. Bare, et al., “Specific Substitution into the Anticodon Loop of Yeast Tyrosine Transfer RNA”, Biochemistry 1986, 25, pp. 5825-5830; 1986 American Chemical Society.
A. Gregory Bruce, et al., “Reactions at the terminal tRNA with T4 RNA ligase”, Nucleic Acids Research, vol. 5, No. 10, Oct. 1978, pp. 3665-3677.
Paul J. Romanuk, et al., “Joining RNA Molecules with RNA Ligase”, Methods in Enzymology, vol. 100 pp. 52-59. XP001248219, 1983.

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