Methods and systems for image processing of microfluidic...

Details Methods and systems for image processing of microfluidic... Methods and systems for image processing of microfluidic...

2007-09-13

2011-11-08

Vanore, David A (Department: 2881)

Image analysis

Applications

Biomedical applications

C382S133000, C382S168000, C382S195000, C382S199000, C382S276000, C422S050000, C422S504000, C435S005000, C435S086000

Reexamination Certificate

active

08055034

ABSTRACT:
A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

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