Recombinant DNA, process for the production thereof and the use

Details Recombinant DNA, process for the production thereof and the use Recombinant DNA, process for the production thereof and the use

1988-05-09

1991-12-24

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4353201, 536 27, C12N 1500, C12N 1511, C12P 2100

Patent

active

050752235

ABSTRACT:
The present invention provides a recombinant DNA, wherein it contains DNA sequences coding for the N-terminal membrane-penetrating domain of the E-protein of the phage .phi.X 174 and DNA sequences coding for the C-terminal membrane-penetrating domain of the L-protein of the phage MS2 and the DNA sequences of both phages are connected by a DNA sequence coding for a hydrophilic flexible amino acid sequence.
The present invention also provides a process for the production of this recombinant DNA.
Furthermore, the present invention provides for the use of the recombinant DNA and of a plasmid containing it for obtaining eukaryotic and prokaryotic metabolic products and gene-technologically produced proteins.

REFERENCES:
patent: 4595658 (1986-06-01), Zinder et al.
Berkhout et al.; EMBO J. 4: 3315 (1985).
Buckley et al.; Mol. Gen. Genet. 204: 120 (1986).
Barrell et al.; Nature 264: 34 (1976).
Harkness et al., FEMS Microbiol. Lett 48: 19-24 (1987).
Berkhout et al., Gene 37: 171-179 (1985).
Blasi et al., J. Gen. Virol 66: 1209-1213 (1985).

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