Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1989-03-27
1991-12-24
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 77, 4351723, 536 27, C12G 170, C12P 1158, C12N 1162
Patent
active
050752120
ABSTRACT:
The present invention discloses methods for detecting picornaviruses in biological tissues. In the methods of the invention, at least a portion of picornavirus nucleic acid present in a test sample of a biological tissue suspected of containing picornavirus is amplified. Picornavirus nucleic acid is then detected by conventional separation techniques or by hybridizing the amplified nucleic acid with at least a portion of a nucleotide probe comprising a nucleotide sequence complementary to the amplified nucleic acid and detecting the probe. The invention also provides nucleotide primers and probes having nucleotide sequences characteristic of picornaviral RNA for use in detecting the viruses.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis
patent: 4683202 (1987-07-01), Mullis
Rotbart et al., J. Clin. Microbiol. 20:1105-1108, (1984).
Rotbart et al., J. Clin, Microbiol 22:220-224, (1985).
Rotbart and Levin, Chapter 15, "Progress Toward the Development of a Pan-Enteroviral Nucleic Acid Probe", in DNA Probes for Infectious Diseases, pp. 193-209, 197.
Rotbart et al., Molecular and Cellular Probes 2:65-73, (1988).
Eur. J. Immunol. vol. 18, issued 1988 Brueggemann et al., "Sequence of a Rat Immunoglobulin .gamma.2.sub.c Heavy Chain Constant Region cDNA: Extensive Homology to Mouse .gamma..sub.3 ".
FEBS Lett., vol. 192; issued 1985, Chong, et al., "Structural Analysis of a New GC Specific Insertion Element IS186".
Virology, vol. 156, issued 1987, Iizuka et al., "Complete Nucleotide Sequence of the Genome of Coxsackievirus B1".
Journal of Clinical Microbiology, issued Dec. 1988, Rotbart et al., "Nonisotopic Oligomeric Probes for the Human Enteroviruses".
Orkin, Stuart H., "Genetic Diagnosis by DNA Analysis", The New England Journal of Medicine, vol. 317, No. 16, pp. 1023-1025.
Schochetman, G. et al., "Polymerase Chain Reaction", The Journal of Infectious Diseases, vol. 158, No. 6, Dec. 1988, pp. 1154-1157.
Saiki, R. K. et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science Reports, vol. 239, Jan. 29, 1988, pp. 487-491.
Abbott, M. A. et al., "Enzymatic Gene Amplification: Qualitative and Quantitative Methods for Detecting Proviral DNA Amplified in Vitro", The Journal of Infectious Diseases, vol. 158, No. 6, Dec. 1988, pp. 1158-1166.
Mullis, K. B. et al., "Specific Synthesis of DNA in Vitro Via A Polymerase Catalysed Chain Reaction", Methods In Enzymology, 155:335-350, 1987.
Mack, D. H. et al., "A Sensitive Method For The Identification Of Uncharacterized Viruses Related To Known Virus Groups: Hepadnavirus Model System", Proc. Natl. Acad. Sci. U.S.A., vol. 85, pp. 6977-6981, Sep. 1988.
Rigby, Peter W. J., et al., "Labeling Deoxyribonucleic Acid to High Specific Activity in vitro by Nick Translation with DNA Polymerase I", J. Mol. Biol. (1977).
Tchen, Paul, "Chemical Labeling of Nucleic Acids: An Alternative to the Use of Radioactive Isotopes", Unite de Recherches de Genetique Epidemiologique, INSERM U. 155, p. 93.
Ruth, Jerry L., "Chemical Synthesis of Non-Radioactively-Labeled DNA Hybridization Probes", Molecular Biosystems, Inc., p. 123.
Lindberg, A. Michael, et al., "Genome of Coxsackievirus B3", Virology 156, 50-63 (1987).
Denhart, David T., "A Membrane-Filter Technique for Detection of Complementary DNA", Biochemical and Biophysical Research Communications, vol. 23, No. 5, 1966.
Toyoda, Haruka, et al., "Complete Nucleotide Sequences of all Three Poliovirus Serotype Genomes; Implication For Genetic Relationship, Gene Function and Antigenic Determinants", J. Mol. Biol. (1984) 174, 561-585.
Jenkins, O., et al., "The Complete Nucleotide Sequence of Coxsackievirus B4 and Its Comparison to Other Members of the Picornaviridae", J. Gen. Virol. (1987), 68, 1835-1848.
Maniatis, T. et al., "Rapid End-labeling of DNA", Molecular Cloning (1982), pp. 117, 127.
Sisson Bradley L.
University of Patents, Inc.
Wax Robert A.
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