Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-05-18
2011-12-20
Babic, Christopher M. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
08080375
ABSTRACT:
An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample.
REFERENCES:
patent: 5049490 (1991-09-01), Sutherland et al.
patent: 5436143 (1995-07-01), Hyman
patent: 5516663 (1996-05-01), Backman et al.
patent: 6100028 (2000-08-01), Cole et al.
patent: 6238864 (2001-05-01), Yan
patent: 6548268 (2003-04-01), Rambach
patent: 6642000 (2003-11-01), Strizhkov et al.
patent: 6753177 (2004-06-01), Stocker et al.
patent: 2005/0118665 (2005-06-01), Zhou et al.
patent: 0 599 270 (1994-06-01), None
patent: WO 2005/012567 (2005-02-01), None
Anonymous: Internet Article; XP002398001; URL:http://www.bdbiosciences.com
vCategory.jsp?action=SELECT&form=formTree—catBean&item=112351>.
Banin, S. et al.: “Demonstration of an Alternative Approach to Immuno-PCR” Clinical Chemistry, American Association for Clinical Chemistry, vol. 50, No. 10, Oct. 2004, pp. 1932-1934, XP001203947.
Niemeyer C M et al. “Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification”, Trends in Biotechnology, vol. 23, No. 4, Apr. 2005.
Banin, S. et al. “Indirect immuno-PCR (i2PCR). Development of a simplified ‘one step’ protocol.” Poster presented at AACC Oak Ridge Conference, San Jose, CA, Apr. 2006.
Office Action issued on Jun. 4, 2010 for EP Application No. 06727136.1.
Claims currently pending in EP Application No. 06727136.1 upon which the Office Action dated Jun. 4, 2010 was based.
Banin Sharon
Stanley John Christopher
Wilson Stuart
Babic Christopher M.
Iseao Technologies Limited
Lucas & Mercanti LLP
LandOfFree
Methods and kits for detecting an enzyme capable of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and kits for detecting an enzyme capable of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and kits for detecting an enzyme capable of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4272460