Particle analysis assay for biomolecular quantification

Details Particle analysis assay for biomolecular quantification Particle analysis assay for biomolecular quantification

2006-02-06

2011-10-11

Switzer, Juliet C (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

08034555

ABSTRACT:
A method is provided for carrying out multi-step separations of objects bearing at least two binding sites. In the first step, a first binder/bead composition is bound to objects that bear the first binding site, and then unbound objects, i.e. objects not bearing the first binding site, are separated from bound objects. In the second step, a second binder/bead composition is bound to the remaining objects that bear the second binding site, and then the objects that are bound to both beads are removed from those objects that are bound to only one bead. The beads can differ in magnetic responsiveness, charge, size, color, and the like, and these differences can be used to carry out the separation steps.

REFERENCES:
patent: 4812401 (1989-03-01), Tarnowski
patent: 4861705 (1989-08-01), Margel
patent: 4935147 (1990-06-01), Ullman
patent: 5145784 (1992-09-01), Cox
patent: 5279936 (1994-01-01), Vorpahl
patent: 5447841 (1995-09-01), Gray
patent: 5487970 (1996-01-01), Rowley
patent: 5731153 (1998-03-01), Lucas et al.
patent: 5736330 (1998-04-01), Fulton
patent: 5741650 (1998-04-01), Lapidus et al.
patent: 5783387 (1998-07-01), Lucas et al.
patent: 6027879 (2000-02-01), Lucas et al.
patent: 0304845 (1989-03-01), None
patent: 5281230 (1993-10-01), None
patent: 5281230 (1993-10-01), None
patent: WO-86/07387 (1986-12-01), None
patent: WO 92/19775 (1992-11-01), None
patent: WO-00/55363 (2000-09-01), None
Hakala et al. (Bioconjugate Chem. 1998, 9, 316-321).
Technical Data Sheet 431. Fluoresbrite Microparticles. Polysciences, Inc. 2009. three pages.
Vlieger, A.M., et al. “Quantitation of Polymerase Chain Reaction Products by Hydbridization-Based Assays with Fluorescent, Colormetric, or Chemiluminescent Detection,” Analytical Biochemistry, Aug. 15, 1992, pp. 1-7, vol. 205.
Ahern, H., “Biochemical, Reagent Kits Offer Scientists Good Return on Investment,” The Scientist, Jul. 1995, p. 20, vol. 9, No. 15.
Blackburn GF, Shah HP, Kenten JH, Leland J, Kamin RA, Link J, Peterman J, Powell MJ, Shah A, Talley DB, et al. (1991) Electrochemiluminescence detection for development of immunoassays and DNA probe assays for clinical diagnostics.Clin Chem. 37(9): 1534-39.
Delfau MH, Kerckaert JP, Collyn d'Hooghe M, Fenaux P, Lai: JL, Jouet JP, Grandchamp B. (1990) Detection of minimal residual disease in chronic myeloid leukemia patients after bone marrow transplantation by polymerase chain reaction. Leukemia. 4(1): 1-5.
Deloukas P, Schuler GD, Gyapay G, Beasley EM, Soderlund C, et al. (1998) A physical map of 30,000 human genes. Science. 282(5389): 744-46.
Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM. (1999) Expression profiling using cDNA microarrays. Nat Genet. 21(1 Suppl): 10-14.
Evans HJ, Buckton KE, Sumner AT. (1971) Cytological mapping of human chromosomes: results obtained with quinacrine fluorescence and the acetic-saline-Giemsa techniques. Chromosoma. 35(3): 310-25.
Langlois RG, Bigbee WL, Kyoizumi S, Nakamura N, Bean MA, Akiyama M, Jensen RH. (1987) Evidence for increased somatic cell mutations at the glycophorin a locus in atomic bomb survivors. Science. 236(4800): 445-48.
Lucas JB, Awa A, Straume T, Poggensee M, Kodama Y, Nakano M, Ohtaki K, Weier HU, Pinkel D, Gray J, et al. (1992) Rapid translocation frequency in analysis in humans decades after exposure to ionizing radiation. Int J Radiat Biol. 62(1): 53-63.
Ninfa A, Ballou DP. (1998) Fundamental Laboratory Approaches for Biochemistry and Biotechnology, pp. 102-103 (Bethesda, MD: Fitzgerald Science Press, Inc.).
Ottewill RH, Shaw JN. (1967) Studies on the Preparation and Characterisation of Monodisperse Polystyrene Latices. Part 2. Electrophoretic Characterisation cf Surface Groupings. Kolloid Z.u.Z Polymere. 218: 34-40.
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J. (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA. 85(23): 9138-42.
Pinkel D, Straume T, Gray JW. (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA. 83(9): 2934-38.
Sandberg AA. (1979) the Chromosome in Human Cancer and Leukemia, pp. 69-73 (New York: Elsevier).
Solomon E, Borrow J, Goddard AD. (1991) Chromosome aberrations and cancer. Science. 254(5035): 1153-60.
Speicher MR, Petersen S, Uhrig S, Jentsch I, Fauth C, Eils R, Petersen I. (2000) Analysis of chromosomal alterations in non-small cell lung cancer by multiplex-FISH, comparative genomic hybridization, and multicolor bar coding. Lab Invest. 80(7): 1031-41.
Stratagene Catalog. (1988), Gene Characterization Kit, p. 39.
Straume T, Lucas J. “Validation studies for monitoring of workers using molecular cytogenetics” in Biomarkers in Occupational Health: Progress and Perspectives (Mendelsohn ML, Peeters JP, and Normandt MJ, Eds.), pp. 174-193 (1995).
Straume T, Lucas JN, Tucker JD, Bigbee WL, Langlois RG. (1992) Biodosimetry for a radiation worker using multiple assays. Health Phys. 62(2): 122-30.
Straume T, Lucas A. (1993) A comparison of the yields of translocations and dicentrics measured using fluorescence in situ hybridization. Int J Radiat Biol. 64(2):185-87.
Tkachuk DC, Westbrook CA, Andreeff M, Donlon TA, Cleary ML, Suryanarayan K, Homge M, Redner A, Gray J, Pinkel D. (1990) Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization. Science. 250(4980): 559-62.
Vooijs M, Yu LC, Tkachuk D, Pinkel D, Johnson D, Gray JW. (1993) Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR. Am J Hum Genet. 52(3): 586-97.
Zippelius A, Pantel K. (2000) RT-PCR-based detection of occult disseminated tumor cells in peripheral blood and bone marrow of patients with solid tumors. An overview. Ann N Y Acad Sci. 906: 110-23.
International Preliminary Examination Report issued Mar. 18, 2002 for PCT/US2000/027883 filed Oct. 10, 2000, which published as WO 2000/027328 on Apr. 19, 2001 (Inventors—Straume et al.; Applicants—University of Utah Research Foundation).
Revised Internationasl Search Report issued Apr. 22, 2002 for PCT/US2000/027883 filed Oct. 10, 2000, which published as WO 2000/027328 on Apr. 19, 2001 (Inventors—Straume et al.; Applicants—University of Utah Research Foundation).
Original International Search Report issued Jan. 18, 2001 for PCT/US2000/027883 filed Oct. 10, 2000, which published as WO 2000/027328 on Apr. 19, 2001 (Inventors—Straume et al.; Applicants—University of Utah Research Foundation).
Certification of Correction issued Oct. 17, 2006 for U.S. Patent No. 6,994971, which issued on Feb. 7, 2006 (formerly U.S. Appl. No. 10/089,560 filed on Apr. 1, 2002) (Inventors—Straume et al.).
Request for a Certification of Correction filed Jul. 25, 2006 for U.S. Patent No. 6,994,971, which issued on Feb. 7, 2006 (formerly U.S. Appl. No. 10/089,560 filed on Apr. 1, 2002) (Inventors—Straume et al.).
Notice of Allowance and Fee(s) Due issued May 31, 2005 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Amendment and Response to Final Office Action filed May 11, 2005 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Final Office Action issued Nov. 12, 2004 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Amendment and Response to Office Action filed Sep. 10, 2004 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Non-Final Office Action issued Jul. 22, 2004 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Response to Restriction Requirement filed May 28, 2004 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).
Restriction Requirement issued Dec. 1, 2003 for U.S. Appl. No. 10/089,560, which as filed on Apr. 1, 2002 (Inventors—Straume et al.).

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