Method for the construction of randomized gene sequence...

Details Method for the construction of randomized gene sequence... Method for the construction of randomized gene sequence...

2003-04-11

2010-11-16

Joike, Michele K (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S007100, C435S069100, C435S254200, C435S320100

Reexamination Certificate

active

07833703

ABSTRACT:
An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using aKluyveromyces lactiskiller toxin, in particular the (γ-subunit of theK. lactiskiller toxin, as negative selection marker is described. The use of the (γ-subunit ofK. lactisas negative selectable marker increases the percentage of randomized clones.

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International Search Report dated Aug. 5, 2003 for corresponding PCT application No. PCT/IB03/01452.
International Preliminary Examination Report dated Aug. 6, 2005 for corresponding PCT application No. PCT/IB03/01452.

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