Method for stabilization of proteins in solution

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S007910, C435S007920

Reexamination Certificate

active

07745145

ABSTRACT:
The present invention relates to a method for stabilization of analytes in solutions of solubilized body samples. The method comprises the steps of solubilizing the body samples obtained from a subject in a suitable sample medium and stabilizing said body sample contained within the sample medium by heating said sample medium for a certain period of time. A further aspect of the invention is a method of a denaturing immunoassay of proteins in solution. The method comprises the steps of bringing a sample containing proteins into contact with a denaturing agent and heating said sample in the presence of the denaturing agent to allow the protein to be denatured.

REFERENCES:
patent: 5654145 (1997-08-01), Fukuda
patent: 5686073 (1997-11-01), Campbell et al.
patent: 5965454 (1999-10-01), Farmilo et al.
patent: 6043415 (2000-03-01), Strizhov et al.
patent: 7306926 (2007-12-01), Doeberitz et al.
patent: 2002/0039583 (2002-04-01), Subjeck et al.
patent: 2004/0132113 (2004-07-01), Nagy
patent: 2005/0048602 (2005-03-01), Takeuchi et al.
patent: 2005/0084924 (2005-04-01), Shults et al.
patent: 2007/0037207 (2007-02-01), Tachikawa et al.
patent: 4239232 (1994-05-01), None
patent: 1388734 (2004-11-01), None
patent: 0185870 (1986-07-01), None
patent: WO 03005766 (2003-12-01), None
Kella N K D et al: “Effect of cetyltrimethylammonium bromide on the heat-induced denaturation and protein-protein interactions of arachin at pH 3.6”, Int. Journal of Peptide and Protein Research, vol. 26, No. 2, 1985, pp. 179-186, XP009069323, abstract, pp. 179-181.
Kella N K D et al: “Effect of sodium dodecyl sulfate on the heat denaturation and aggregation of arachin at pH 3.6” Int. Journal of Peptide and Protein Research, vol. 25, No. 3, 1985, pp. 308-315, XP009069322, pp. 308-310.

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