Protein refolding agent and refolding method

Details Protein refolding agent and refolding method Protein refolding agent and refolding method

2006-08-11

2010-12-28

Shiao, Rei-tsang (Department: 1628)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Radical -xh acid, or anhydride, acid halide or salt thereof...

C562S512000

Reexamination Certificate

active

07858661

ABSTRACT:
A refolding agent and refolding method which make it possible to produce high-purity proteins in high productivity. The refolding agent includes a phosphorus-containing compound (A) and an oxycarbonyl group-containing compound (B). The refolding method includes the step of treating the unfolded protein with the refolding agent. As the compound (A), there may be mentioned at least one species selected from inorganic phosphoric acids, alkyl phosphate esters, sugar phosphate esters, and salts of these, and as the compound (B), there may be mentioned at least one species selected from formic acid, acetic acid, propionic acid, lactic acid, tartaric acid, and salts of these.

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