Detection of mycobacteria

Details Detection of mycobacteria Detection of mycobacteria

1994-08-05

1996-12-10

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, C12Q 168, C12Q 170

Patent

active

055829856

ABSTRACT:
The invention provides a method, compositions, and kits useful for detecting mycobacteria in a sample. The method includes contacting the sample with a formaldehyde solution, an organic solvent, and a protein-degrading agent prior to hybridizing a mycobacteria-specific nucleic acid probe to the sample. The invention has particular utility in detection and susceptibility screening of human-disease causing mycobacteria such as Mycobacterium tuberculosis.

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