Method for determination of amount of double-stranded DNA...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S022100, C536S023100, C536S024300

Reexamination Certificate

active

07851160

ABSTRACT:
A method and kit for electrochemically simply determining with excellent precision the amount of double-stranded DNA does not require an expensive measurement electrode, such as an immobilized enzyme electrode or any high level electrode production technique which can retain uniform surface area accuracy. A method and kit are provided for electrochemically determining the amount of double-stranded DNA in a sample solution based on a residual amount of a substance capable of binding to the double-stranded DNA which is added to the solution in excess amount, in which a buffering substance is added to a sample solution, the buffering substance being capable of allowing an oxidation wave potential of the potential-current curve for the substance capable of binding to the double-stranded-DNA determined in a solution containing the buffering substance to change depending on the concentration of the free substance capable of binding to the double-stranded DNA in the solution.

REFERENCES:
patent: 6365346 (2002-04-01), Patel et al.
patent: 6664047 (2003-12-01), Haugland et al.
patent: 2002-504350 (2002-02-01), None
patent: 2002-218998 (2002-08-01), None
patent: 2004-257993 (2004-09-01), None
patent: 2004-257993 (2004-09-01), None
Malatesta, F. et al: “Potentiometric Characterization of Ethidium Bromide and of Its Reactions With Nucleic Acids” Analytical Biochemistry, Academic Press Inc, New York, vol. 334, No. 1, Nov. 1, 2004, pp. 62-71, XP004583734 ISSN: 0003-2697.
Heli, H. et al: “An Electrochemical Study of Neutral Red-DNA Interaction” Electrochimica Acta, Elsevier Science Publishers, Barking, GB, vol. 51, No. 6, Jul. 15, 2005, pp. 1108-1116, XP005161523 ISSN: 0013-4686.
Maruyama, K. et al: “Detection of Target DNA by Electrochemical Method” Sensors and Actuators B, Elsevier Sequoia S.A., Lausanne, CH, vol. 76, No. 1-3, Jun. 1, 2001, pp. 215-219, XP004241120 ISSN: 0925-4005.
International Search Report dated Aug. 27, 2009.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for determination of amount of double-stranded DNA... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for determination of amount of double-stranded DNA..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determination of amount of double-stranded DNA... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4228514

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.