Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-10-16
1999-03-02
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 43525411, 4353201, 435325, 435419, 536 236, C12P 2100, C12N 1529, C12N 1563, C12N 510
Patent
active
058769641
ABSTRACT:
A cDNA encoding geranyl diphosphate synthase from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID No:1) is provided which codes for the expression of geranyl diphosphate synthase (SEQ ID No:2) from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase or for a base sequence sufficiently complementary to at least a portion of the geranyl diphosphate synthase DNA or RNA to enable hybridization therewith (e.g., antisense geranyl diphosphate synthase RNA or fragments of complementary geranyl diphosphate synthase DNA which are useful as polymerase chain reaction primers or as probes for geranyl diphosphate synthase or related genes). In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase that may be used to facilitate the production, isolation and purification of significant quantities of recombinant geranyl diphosphate synthase for subsequent use, to obtain expression or enhanced expression of geranyl diphosphate synthase in plants in order to enhance the production of monoterpenoids, to produce geranyl diphosphate in cancerous cells as a precursor to monoterpenoids having anti-cancer properties or may be otherwise employed for the regulation or expression of geranyl diphosphate synthase or the production of geranyl diphosphate.
REFERENCES:
Croteau, R. and Purkett, P.T., "Geranyl Pyrophosphate Synthase: Characterization of the Enzyme and Evidence That This Chain-Length Specific Prenyltransferase is Associated with Monoterpene Biosynthesis in Sage (Salvia officinalis)," Arch Biochem. Biophys., 271(2):524-535 (1989).
Heide, L. and Berger, U., "Partial Purification and Properties of Geranyl Pyrophosphate Synthase from Lithospermum erythrorhizon Cell Cultures," Arch. Biochem Biophys., 273(2):331-338 (1989).
Suga, T. and Endo, T., Geranyl Diphosphate Synthase in Leaves of Pelargonium Roseum, Phytochemistry, 30(6):1757-1761 (1991).
Clastre, M. et al., "Purification and Characterization of Geranyl Diphosphate Synthase from Vitis vinifera L. cv Muscat de Frontignan Cell Cultures," Plant Physiol.,102:205-211 (1993).
Scolnik, P.A. and Bartley, G.E., "A Table of Some Cloned Plant Genes Involved in Isoprenoid Biosynthesis," Plant Mol. Biol. Reporter,14(4):305-319 (1996).
Burke Charles Cullen
Croteau Rodney Bruce
Gershenzon Jonathan
Wildung Mark Raymond
Brusca John S.
Degen Nancy
Washington State University Research Foundation
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