Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-07-27
2010-06-29
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007210, C435S007240, C435S286500, C435S287200, C436S517000, C436S546000, C436S010000, C436S056000, C436S063000, C436S064000, C436S164000, C436S172000, C424S009100, C422S073000, C422S082080, C422S082090
Reexamination Certificate
active
07745155
ABSTRACT:
The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in-vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest. In some embodiments, the circulating cells comprise apoptotic cells whose detection can allow, e.g., non-invasive monitoring of the efficacy of a cancer treatment, such as an anti-tumor or an anti-angiogenic therapy.
REFERENCES:
patent: 5644388 (1997-07-01), Maekawa et al.
patent: 5976502 (1999-11-01), Khoobehi et al.
patent: 6337920 (2002-01-01), Muhlhoff
patent: 6462345 (2002-10-01), Simon et al.
patent: 6507400 (2003-01-01), Pina et al.
patent: 6548796 (2003-04-01), Silvermintz et al.
patent: 6549275 (2003-04-01), Cabuz et al.
patent: 6554775 (2003-04-01), Peyman et al.
patent: 6589792 (2003-07-01), Malachowski
patent: 6597438 (2003-07-01), Cabuz et al.
patent: 6646742 (2003-11-01), Gangstead et al.
patent: 6687052 (2004-02-01), Wilson et al.
patent: 6727071 (2004-04-01), Dunlay et al.
patent: 6811983 (2004-11-01), Sugden et al.
patent: 7264794 (2007-09-01), Georgakoudi et al.
patent: 7491502 (2009-02-01), Lin
patent: 2005/0101524 (2005-05-01), Hogg
patent: 2006/0134002 (2006-06-01), Lin
patent: 2006/0134005 (2006-06-01), Lin et al.
patent: 2007/0111225 (2007-05-01), Lambert et al.
patent: 2007/0249734 (2007-10-01), Gilbert et al.
patent: 2007/0274919 (2007-11-01), Dertinger
patent: WO-9834536 (1998-08-01), None
Novak et al., In vivo flow cytometer for real-time detection and quantification of circulating cells, Optical Letters, 29 (1): 77-79 (Jan. 1, 2004).
Georgakoudi et al., In Vivo Flow Cytometer: A New Method for Enumerating Circulating Cancer cells, Cancer Research, 64: 5044-5047 (Aug. 1, 2004).
Petrovsky et al., Near-Infrared Fluorescent Imaging of Tumor Apoptosis, Cancer Research 63: 1936-1942 (Apr. 15, 2003).
Yamamoto et al., Granulocytes from patients with paroxysmal nocturnal hemoglobinuria and normal individuals have the same sensitivity to spontaneous apoptosis, Experimental Hematology 30: 187-194 (2002).
Carbonari et al., Detection and Characterization of Apoptotic Peripheral Blood Lymphocytes in Human Immunodeficiency Virus Infection and Cancer Chemotherapy by a Novel Flow Immunocytometric Method, Blood 83 (5): 1266-1277 (Mar. 1, 1994).
Database BIOSIS, Accession No. 2005; 535990, Buller G. Monomeric Cyanine dye permeability correlated with annexin-V staining on apoptotic cells using flow cytometry. FASEB Journal, Mar. 7, 2005, vol. 19, No. 5, Suppl. S, Part 2, pp. A1673 Abstract.
Database BIOSIS, Accession No. 2002: 2780, Nicholas, A.P. In vitro neuronal cell death mediated by alpha-1 A/D adrenoceptors. Society of Neuroscience Abstracts. 2001, vol. 27, No. 2, pp. 2141. Abstract.
Aotake, T., et al., “Changes of angiogenesis and tumor cell apoptosis during colorectal carcinogenesis”, 1999,Clin Cancer Res5(1):135-142.
Bedner, E., et al., “High affinity binding of fluorescein isothiocyanate to esosinophils detected by laser scanning cytometry: a potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry”, 1999,Cytometry36(1):77-82.
Blankenberg, F.G., et al., “Imaging cyclophosphamide-induced intramedullary apoptosis in rats using 99mTc-cradiolabeled annexin”, 2001,Journal of Nuclear Medicine42(2):309-316.
Brooks, P.C., et al., “Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels”, 1994,Cell79(7):1157-1164.
Carbonari, M., et al., “Detection and characterization of apoptotic peripheral blood lymphocytes in human immunodeficiency virus infection and cancer chemotherapy by a novel flow immunocytometric method”, 1994,Blood83(5):1268-1277.
Cummings, M.C., “Apoptosis”, 1997,Am J Surg Pathol21(1):88-101.
Durrieu, F., et al., “Caspase activation is an early event in anthracycline-induced apoptosis and allows detection of apoptotic cells before they are ingested by phagocytes”, 1998a,Exp Cell Res240(2):165-175.
Ek, O., et al., “Treatment of human B-cell precursor leukemia in SCID mice by using a combination of the anti-CD19 immunotoxin B43-PAP with the standard chemotherapeutic drugs vincristine, methylprednisolone, and L-asparaginase”, 1998a,Leuk Lymphoma31(1-2):143-149.
Ek, O., et al., “Combined therapeutic efficacy of the thymidylate synthase inhibitor ZD1694 Tomudex) and the immunotoxin B43(anti-CD19)-PAP in a SCID mouse model of human B-lineage acute lymphoblastic leukemia”, 1998b,Leuk Lymphoma28(5-6):509-514.
Fadeel, B., “Apoptosis in human disease: a new skin for the old ceremony?”, 1999,Biochem Biophys Res Commun266(3):699-717.
Gaiano, N., “A method for rapid gain-of function studies in the mouse embryonic nervous system”, 1999,Nat Neurosci2(9):812-819.
Gaiano, N., “Radial glial identity is promoted by Notch1 signaling in the murine forebrain”, 2000,Neuron26(2):395-404.
Georgakoudi, I., “in vivo flow cytometry: a new method of enumerating circulating cancer cells”, 2004,Cancer Res64(15);5044-5047.
Herschman, H.R., “Molecular imaging: looking at problems, seeing solutions”, 2003, Science 302(5645):605-608,
Holdenrieder, S., “Apoptotic markers in cancer”, 2004,Clin Biochem37(7):605-617.
Jarh, S., “DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells”, 2001,Cancer Res61(4):1659-1665.
Johnston, J.B., et al., “Induction of apoptosis in CD4+ prolymphocytic leukemia by deoxyadenosine and 2′-deoxycoformycin”, 1992,Leukemia Research16(8):781-788.
Laxman, B., “Noninvasive real-time imaging of apoptosis”, 2002,Proceedings of the National Academy of Sciences of the United States of America99(26):16551-16555.
Massoud, T.F., et al., “Molecular imaging in living subjects: seeing fundamental biological processes in a new light”, 2003,Genes&Development, 17(5):545-580.
Matsubara, K, et al., “Induction of apoptosis in childhood acute leukemia by chemotherapeutic agents: failure to detect evidence of apoptosis in vivo”, 1994,European Journal of Haematology52(1):47-52.
Nery, S., et al., “Sonic hedgehog contributes to oligodendrocyte specification in the mammalian forebrain”, 2001,Development128(4):527-540.
Novak, J., et al., “In vivo flow cytometer for real-time detection and quantification of circulating cells”, 2004,Opt Lett29(1):77-79.
Ntziachristos, V., et al., “Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V-Cy5.5 conjugate”, 2004,Proc Natl Acad Sci USA101(33):12294-12299.
Osella-Abate, S., et al., “Expression of apoptosis markers on peripheral blood lymphocytes from patients with cutaneous T-cell lymphoma during extracorporeal photochemotherapy”, 2001,J Am Acad Dermatol44(1):40-47.
Petrovsky, A., et al, “Near-infrared fluorescent imaging of tumor apoptosis”, 2003,Cancer Research63(8):1936-1942.
Reed, J., “Dysregulation of apoptosis in cancer”, 1999,J Clin Oncol17(9):2941-2953.
Rich, T., et al., “Defying death after DNA damage”, 2000,Nature407(6805):777-783.
Saito, T., et al., “Spontaneous ex vivo apoptosis of peripheral blood mononuclear cells in patients with head and neck cancer”, 1999,Clinical Cancer Research5(6):1263-1273.
Savill, J., et al., “Corpse clearance defines the meaning of cell death”, 2000,Nature407(6805):784-788.
Schellenberger, E.A., et al., “Optical imaging of apoptosis as a biomarker of tumor response to chemot
Lin Charles P.
Sipkins Dorothy
Wei Xunbin
Gabel Gailene R
Massachusetts General Hospital
Nutter & McClennen & Fish LLP
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