Reagent and process for the determination of hemoglobin in blood

Details Reagent and process for the determination of hemoglobin in blood Reagent and process for the determination of hemoglobin in blood

1987-04-10

1989-01-24

Marcus, Michael S.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

G01N 3372

Patent

active

048001678

ABSTRACT:
A reagent system for determing the hemoglobin content of whole blood comprising an aqueous solution of polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 at a pH greater than about 8.0 to denature hemoglobin in whole blood. A method is also provided wherein a blood sample is treted with an aqueous solution of polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 at a basic pH sufficient to denature the hemoglobin present in the whole blood to form a stable product whereafter the absorbance of the stable product of the blood sample and polyvinylpyrrolidone of molecular weight from about 10,000 to about 360,000 polymer reagent system is measured at a wavelength of approximately 575 nm to determine the hemoglobin contentration.

REFERENCES:
patent: 4341527 (1982-07-01), Zander et al.
patent: 4673654 (1987-06-01), Talmage
Lyonnais et al., J. Lab. Clin, Med., (85)2, pp. 329-336 (1975).

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