Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-09-30
2010-06-01
Riley, Jezia (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S026600, C548S469000
Reexamination Certificate
active
07727716
ABSTRACT:
The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.
REFERENCES:
patent: 4883867 (1989-11-01), Lee et al.
patent: 4957870 (1990-09-01), Lee et al.
patent: 5321130 (1994-06-01), Yue
patent: 5436134 (1995-07-01), Haugland et al.
patent: 5534416 (1996-07-01), Millard et al.
patent: 5582702 (1996-12-01), Cabilly et al.
patent: 5656449 (1997-08-01), Yue
patent: 5658751 (1997-08-01), Yue et al.
patent: 5863753 (1999-01-01), Haugland et al.
patent: 5865874 (1999-02-01), Trainer
patent: 5929227 (1999-07-01), Benson
patent: 6379516 (2002-04-01), Cabilly et al.
patent: 6562213 (2003-05-01), Cabilly et al.
patent: 2002/0112960 (2002-08-01), Cabilly et al.
patent: 2002/0134680 (2002-09-01), Cabilly et al.
patent: WO 94/24213 (1994-10-01), None
patent: WO 96/34276 (1996-10-01), None
patent: WO 97/41070 (1997-11-01), None
patent: WO 99/42620 (1999-08-01), None
Waring, M. J. (1965). “Complex formation between ethidium bromide and nucleic acids.”J Mol Biol13(1): 269-82.
McCann, J., E. Choi, et al. (1975). “Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals.”Proc. Natl Acad Sci U S A72(12): 5135-9.
Singer, V. L., T. E. Lawlor, et al. (1999). “Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test).”Mutat Res439(1): 37-47.
Fukunaga, M., B. A. Cox, et al. (1984). “Production of frameshift mutations in Salmonella by phenanthridinium derivatives: enzymatic activation and photoaffinity labeling.”Mutat Res127(1): 31-7.
Le Pecq, J. B. and C. Paoletti (1966). “A new fluorometric method for RNA and DNA determination.”Anal Biochem17(1): 100-7.
Yamasaki, H. (1996). “Use of the Syrian hamster embryo (SHE) cell transformation assay for predicting the carcinogenic potential of chemicals. Introduction.”Mutat Res356(1): 1-3.
Amacher, D. E., S. C. Paillet, et al. (1980). “Point mutations at the thymidine kinase locus in L5178Y mouse lymphoma cells. II. Test validation and interpretation.”Mutat Res72(3): 447-74.
Clive, D., K. O. Johnson, et al. (1979). “Validation and characterization of the L5178Y/TK+/− mouse lymphoma mutagen assay system.”Mutat Res59(1) 61-108.
Maron, D. M. and B. N. Ames (1983). “Revised methods for the Salmonella mutagenicity test.”Mutat Res113(3-4): 173-215.
Clive, D. and J. F. Spector (1975). “Laboratory procedure for assessing specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells.”Mutat Res31(1): 17-29.
Clive, D., W. Caspary, et al. (1987). “Guide for performing the mouse lymphoma assay for mammalian cell mutagenicity.”Mutat Res189(2): 143-56.
Furniss, et al. (eds.),Vogel's Encyclopedia of Practical Organic Chemistry 5thEd., Longman Scientific and Technical Ltd., Essex, 1991, pp. 809-816.
Heller,Acc Chem Res.23: 128 (1990).
PCT/US2004/032693 International Search Report.
Mcintosh, S.L., et al. “Fluorescence lifetime for on-the fly multiplex detection of DNA restriction fragments in capillary electrosphoresis”, Analytical Chemistry, Nov. 1, 2000, vol. 72, No. 21, pp. 5444-5449.
Rye, H.S., et al., “Stable Fluorescent Complexes of Double-stranded DNA with Bis-Intercalating Asymmetric Cyanine Dyes: Properties and Applications”, Nucleic Acids Research, Oxford University Press, Surrey, GB., vol. 20, No. 11, 1992, pp. 2803-2812.
Eriksson, Maja, et al. “Groove-binding unsymmetrical cyanine dyes for staining for DNA: dissociation rates in free solution and electrophoresis gels”, Nucleic Acids Research, Nov. 1, 2003, vol. 31, No. 21, pp. 6235-6242.
Rye, H.S., et al. “High-Sensitivity Two-Color Detection of Double-Stranded DNA with a Confocal Fluorescence Gel Scanner Using Ethidium Homodimer and Thiazole Orange”, Nucleic Acids Research, vol. 19, No. 2, 1991, pp. 327-333.
Timtcheva, I., et al., “New asymmetric monomethine cyanine dyes for nucleic acid labeling: absorption and fluorescence spectral characteristics”, Journal of Photochemistry and Photobiology, A: Chemistry, 120(1), 7-11 Coden: JPPCEJ: ISSN 1010-6030, 2000.
Wilke, WW, et al., “Use of thiazole orange homodimer as an alternative to ethidium bromide for DNA detection in agarose gels.”, Mod Pathol. (Apr. 1994 7(3): 385-7.
Axton, RA, et al., “Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations”, Mod Cell Probes Jun. 1994; 8 (3): 245-50.
Rye, HS, et al., “Picogram detection of stable dye-DNA intercalation complexes with two-color laser-excited confocal fluorescence gel scanner”, Methods Enzymol. 1993; 217:414-31.
Srinivasan, K., Enhanced detection of PCR products through use of TOTO and YOYO intercalating dyes with laser induced fluorescence—capillary electrophoresis. Appl Theor Electrophor. 1993;3(5): 235-9.
Brooker, L.G.S., et al., “Color and Constitution. V. Absorption of Unsymmetrical Cyanines. Resonance as a Basis for a Classification of Dyes”. J. Am. Chem. Soc.; 1942, 64 pp. 199-209.
Quesada, MA, et al., “High-sensitivity DNA detection with a laser-excited confocal fluorescence gel scanner”. Biotechniques. May 1991; 10(5):616-25.
Haab BB, et al., Single molecule fluorescence burst detection of DNA fragments separated by capillary electrophoresis: Ana Chem. Sep. 15, 1995;67(18):3253-60.
Woolley AT, et al., High-speed DNA genotyping using microfabricated capillary array electrophoresis chips. Anal Chem. Jun. 1, 1997;69(11):2181-6.
Skeidsvoll, J., et al., Analysis of double-stranded DNA by capillary electrophoresis with laser-induced fluorescense detection using the monomeric dye SYBR green I. Anal Biochem. Nov. 1, 1995;231(2): 359-65.
Zhu, H., et al. High-sensitivity capillary electrophoresis of double-stranded DNA fragments using monomeric and dimeric fluorescent intercalating dyes. Ana Chem. Jul. 1, 1994;66(13): 1941-8.
Brooker, L.G.S., et al., “Color and Constitution. X. Absorption of the Merocyanines”. J. Am. Chem. Soc.; 1951; 73(11) pp. 5332-5350.
Svanvik, N., et al., “Light-up probes: Thiazole Orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution”. Anal Biochem 281, 26-35 (2000).
Nygren, J., et al., “The Interaction Between the Fluorescent Dye Thiazole Orange and DNA”. Biopolymers, vol. 46, 39-51 (1998).
Ames, Bruce N. et al., “Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test”,Mutati
Beaudet Matthew Paul
Cox W. Gregory
Yue Stephen
Blodgett James K.
Life Technologies Corporation
Life Technologies Corporation
Riley Jezia
LandOfFree
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