Preliophic moleculator using electric fields and gradients...

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical

Reexamination Certificate

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C702S022000, C703S011000, C435S283100

Reexamination Certificate

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07826974

ABSTRACT:
Apparatus and methods of using the apparatus for inducing movement and interaction of molecules in an electric field of an electrophoretic device that is circular in configuration to mimic a living cell. The circular electrophoretic device supports an isoelectric focusing medium, such as a gel on a gel plate with one charged circular pole at the center and an oppositely charged concentric pole at the perimeter of the plate whereupon molecular substances having electronegative or electropositive charges placed in the gel will tend to migrate toward the oppositely charged pole to their isoelectric points, allowing researchers to devise experiments with multiple substances to track pathways of interaction during osmotic diffusion and polar directed migration through the medium that parallel pathways in a biological cell, the device having a thermal control for regulating the temperature of the analytical experiments using the device.

REFERENCES:
patent: 4323439 (1982-04-01), O'Farrell
patent: 4612106 (1986-09-01), Kromer et al.
patent: 4683042 (1987-07-01), Scott
patent: 4810183 (1989-03-01), Place et al.
patent: 5294323 (1994-03-01), Togusari et al.
patent: 5298143 (1994-03-01), Ivory et al.
O'Farrell, P. H., High Resolution Two-Dimensional Electrophoresis of Proteins, The Journal of Biological Chemistry, vol. 250, No. 10, Issue of May 25, pp. 4007-4021, 1975.

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