Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-04-28
2010-10-19
Chin, Christopher L (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007900, C436S518000, C436S809000
Reexamination Certificate
active
07816081
ABSTRACT:
A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.
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Mitsuhashi Masato
Ogura Mieko
Chin Christopher L
Hitachi Chemical Co. Ltd.
Hitachi Chemical Research Center Inc.
Knobbe Martens Olson & Bear LLP
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