MicroRNA molecules

Details MicroRNA molecules MicroRNA molecules

2009-08-31

2010-11-23

Bowman, Amy (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C536S024310, C536S024330, C514S04400A

Reexamination Certificate

active

07838663

ABSTRACT:
InCaenorhabditis elegans, lin-4 and let-7 enclode 22- and 21-nucleotide RNAs, respectively, that function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as “small temporal RNAs” (stRNAs). We show that many more 21- and 22-nt expressed RNAs, termed microRNAs, (miRNAs), exist in invertebrates and vertebrates, and that some of these novel RNAs, similar to let-7 stRAN, are also highly conserved. This suggests that sequence-specific post-transcriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.

REFERENCES:
patent: 5801154 (1998-09-01), Baracchini et al.
patent: 5861310 (1999-01-01), Freeman et al.
patent: 6506559 (2003-01-01), Fire et al.
patent: 6821724 (2004-11-01), Mittman et al.
patent: 6905827 (2005-06-01), Wohlgemuth et al.
Wahlestedt et al., Potent and nontoxic antisense oligonucleotides containing locked nucleic acids, 2000, PNAS, vol. 97, No. 10, pp. 5633-5638.
Krutzfeldt et al., Strategies to determine the biological function of microRNAs, 2006, Nature Genetics, vol. 38, S14-S19.
Cullen, RNAi the natural way, 2005, Nature Genetics, vol. 37, No. 11, pp. 1163-1165.
Lee et al., TheC. elegansHeterochronic Gene lin-4 Encodes Small RNAs with Antisense Complementarity to lin-14, 1993, Cell, vol. 75, pp. 843-854.
Marra et al., AA209594, EST Feb. 18, 1997, see search result labeled “20090122—121332—us-11-747-409-88.rst”, result #3 in SCORE (enclosed in office action).
Lee et al., An Extensive Class of Small RNAs inCaenorhabditis elegans, 2001, Science, vol. 294, pp. 862-864.
Elbashir et al., Functional anatomy of siRNAs for mediating efficient RNAi inDrosophilamelanogaster embryo lysate, 2001, The EMBO Journal, vol. 20, No. 23, pp. 6877-6888.
Lee et al., “An Extensive Class of Small RNAs inCaenorhabditis elegans”, Science, vol. 294, Oct. 26, 2001, pp. 862-864.
Elbashir et al., “Functional anatomy of siRNAs for mediating efficient RNAi inDrosophilamelanogaster embryo lystae”, The EMBO Journal, vol. 20, No. 23, pp. 6877-6888, 2001.
Drosophilamelanogaster sequence (P1 DS08416(D52)), complete sequence; retrieved from Database EMBL Accession No. AC 002442 (Mar. 3, 2000).
Pasquinelli Amy F. et al., “Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA”, Nature, vol. 408, No. 6808, 2000, pp. 86-89.
Reinhart Brenda J. et al., “The 21-nucleotide let-7 RNA regulates developmental timing inCaenorhabditis elegans.”, Nature, vol. 403, No. 6772, Feb. 24, 2000, pp. 901-906.
Moss Eric G. et al., “The cold shock domain protein LIN-28 controls developmental timing inC. elegansis regulated by the lin-4 RNA”., Cell, vol. 88, No. 5, 1997, pp. 637-646.
GenBank accession No. AE014298.
GenBank accession No. AC101777.
GenBank accession No. AL392165.
GenBank accession No. AL355858.
Quintana et al., “Identification of Novel Genes Coding for Small Expressed RNAs”, Science, vol. 294, Oct. 26, 2001, pp. 853-858.
Elbashir et al., “RNA interference is mediated by 21-and 22-nucleotide RNAs”, Genes & Development, 15: 2001, pp. 188-200.

Affiliated with

Also associated with

Rating

MicroRNA molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with MicroRNA molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and MicroRNA molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4153384