Transgenic organisms with lower growth temperature

Details Transgenic organisms with lower growth temperature Transgenic organisms with lower growth temperature

2004-10-11

2010-10-12

Nashed, Nashaat T (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S183000

Reexamination Certificate

active

07811784

ABSTRACT:
The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.

REFERENCES:
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Ferrer et al., “Functional Consequences of Single: Double Ring Transitions in Chaperonins: Life in the Cold,” Blackwell Publishing, Ltd., 2004, Molecular Microbiology. vol. 53, No. 1, pp. 167-182. XP-002337729 (ISR).
Tosco et al., “GroEL From the Psychrophilic Bacterium . . . ,” Extremophiles, 2003, vol. 7, No. 1, pp. 17-28, XP-002337730 (ISR).
Yamauchi et al., “Gene Structure and Transcriptional regulation specific . . . ,” Archives of Microbiology, 2003, vol. 180, No. 4, pp. 272-278. XP-002337731 (ISR).
Yakimov et al., “Oleispira Antarctica Gene . . . ” 2003, International Journal of Systematic and Evolutionary Microbiology, vol. 53, No. 3, pp. 779-785. XP-002337732 (ISR).
Walter et al., “Molecular Chaperones—Cellular . . . ” Angewandte Chemie, 2002, vol. 41, No. 7, pp. 1098-1113. XP-002337733 (ISR).
Ranson et al., “Chaperonins” Biochemical Journal, 1998, vol. 333, No. 2, pp. 233-242. XP-002337734 (ISR).
Ferrer et al., “Chaperonins Govern Growth . . . ” Nature Biotechnology, 2003, vol. 21, No. 11, pp. 1266-1267. XP-009051109 (ISR).
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Médigue et al., “Coping with cold: The genome of the versatile . . . ,” Genome Research, 2005, pp. 1-12.

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