Method, apparatus, reagent kit and reagent for...

Chemistry: analytical and immunological testing – Biological cellular material tested

Reexamination Certificate

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C436S010000, C436S017000, C436S164000, C436S172000, C422S073000, C422S082050, C422S082080, C422S082090, C435S004000, C435S029000

Reexamination Certificate

active

07632683

ABSTRACT:
A method for distinguishing erythrocytes in a biological specimen, includes preparing a sample liquid so as to cause damage to a cell membrane of yeast-like fungi without hemolyzing erythrocytes in a biological specimen and to stain the yeast-like fungi with a fluorescent dye; detecting a first information and a second information from a particle in the sample liquid, wherein the first information reflects a size of the particle and the second information reflects a degree of fluorescent staining of the particle; and distinguishing the erythrocytes from the yeast-like fungi based on the first information and second information detected. An apparatus, a reagent kit and a reagent for carrying out the method are also disclosed.

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Breslin, W.J.; Phillips, J.E.; Lomax, L.G.; Bartels, M.J.; Dittenber, D.A.; Calhoun, L.L; and Miller, R.R. “Hemolytic Activity of Ethylene Glycol Phenyl Ether (EGPE) in Rabbits”;Fundamental and Applied Toxicology, 1991, 17, 466-481.
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