Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-09-08
1996-09-17
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 536 242, 4353201, 4352523, 935 6, 935 8, C07H 2104, C12N 510, C12N 1563
Patent
active
055569552
ABSTRACT:
A process for detection of a new polymorphic locus in a DNA sequence is characterized by the following stages. Polynucleotides are prepared by forming an unspecified oligonucleotide including 5 to 10 bases which are repeated 5 to 100 times. A genomic bank is explored by hybridization under low stringency conditions using the prepared polynucleotides which are labelled. The clones that react with the polynucleotides are analyzed by the Southern method. The restriction fragments detected by the polynucleotides are purified and labelled. The genomic DNA of non-consanguineous individuals is analyzed by the Southern method under highly stringent hybridization conditions, with the obtained restriction fragments. The restriction fragments corresponding to new polymorphic loci are selected.
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Etat Francais represente par le Delegue General pour A'Armement
Jones W. Gary
Myers Carla
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